Detailed information of TA system TA110445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 770442..771092
• Replicon: chromosome
• Accession: NZ_CP031869
• Organism: Actinobacillus pleuropneumoniae strain WF83

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B0BNU3
• Locus tag: D1100_RS03560
• Protein ID: WP_005614926.1
• Coordinates: 770442..770831 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BNU4
• Locus tag: D1100_RS03565
• Protein ID: WP_005604117.1
• Coordinates: 770832..771092 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1100_RS03545 (D1100_03530)	766091..767287	+	1197	WP_012478414.1	amino acid aminotransferase	-
D1100_RS03550 (D1100_03535)	767391..768674	-	1284	WP_005617151.1	Xaa-Pro aminopeptidase	-
D1100_RS03555 (D1100_03540)	769083..770306	-	1224	WP_005617152.1	ATP-binding protein	-
D1100_RS03560 (D1100_03545)	770442..770831	-	390	WP_005614926.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1100_RS03565 (D1100_03550)	770832..771092	-	261	WP_005604117.1	hypothetical protein	Antitoxin
D1100_RS03570 (D1100_03555)	771226..773247	-	2022	WP_005617154.1	excinuclease ABC subunit UvrB	-
D1100_RS03575 (D1100_03565)	773424..774236	+	813	WP_005604121.1	iron uptake system protein EfeO	-
D1100_RS03580 (D1100_03570)	774248..775441	+	1194	WP_237601177.1	iron uptake transporter deferrochelatase/peroxidase subunit	-
D1100_RS03585 (D1100_03575)	775452..776072	+	621	WP_005596976.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14837.17 Da
• Isoelectric Point: 5.1295

>T110445 WP_005614926.1 NZ_CP031869:c770831-770442 [Actinobacillus pleuropneumoniae]
MYMLDTNTVSYFFRQDPTVVKKLQQLNPELICISSVTAAELFYGVKKRNNQKLTAFLNTFLSAITVMDWDYQVAEVYGQL
RAEMEREGKIMGVQDQMIGAHALATECVLVSSDKAFQFIPNLVLENWWK

Nucleotide

• Length: 390 bp

>T110445 NZ_CP031869:c770831-770442 [Actinobacillus pleuropneumoniae]
ATGTATATGCTAGATACTAATACGGTGAGTTATTTCTTTAGGCAAGATCCTACTGTGGTTAAGAAACTACAACAACTTAA
TCCGGAATTGATTTGTATTTCATCGGTCACGGCTGCAGAGTTGTTTTATGGCGTAAAAAAACGTAATAATCAGAAACTTA
CTGCATTTTTAAATACTTTTTTATCTGCGATTACGGTGATGGATTGGGATTATCAAGTGGCGGAAGTTTATGGTCAGCTG
CGAGCAGAGATGGAACGAGAAGGCAAAATCATGGGCGTTCAAGATCAAATGATTGGAGCTCATGCCCTAGCAACAGAATG
TGTTCTTGTTTCCAGCGATAAAGCCTTTCAATTTATCCCTAATCTCGTATTAGAAAATTGGTGGAAGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10289.63 Da
• Isoelectric Point: 5.1867

>AT110445 WP_005604117.1 NZ_CP031869:c771092-770832 [Actinobacillus pleuropneumoniae]
MEKVARLFRNGRNQAVRLPVEFEFNAKQVYVFKEANGDITLSTRSRNQQNWQKVFELLPMLECDEQFLSEQERKQEMATR
DPFEGF

Nucleotide

• Length: 261 bp

>AT110445 NZ_CP031869:c771092-770832 [Actinobacillus pleuropneumoniae]
ATGGAAAAGGTCGCACGTTTATTTAGAAACGGACGTAATCAAGCCGTACGATTACCGGTTGAATTTGAGTTTAATGCGAA
GCAAGTTTATGTGTTTAAAGAAGCAAACGGCGATATTACGCTTTCGACACGTTCTCGCAATCAGCAAAATTGGCAGAAAG
TCTTTGAGTTATTACCAATGCTTGAATGTGATGAACAGTTTTTATCGGAACAAGAACGCAAGCAAGAAATGGCAACTCGA
GATCCGTTCGAGGGCTTTTAA

Structures

Toxin

• AlphaFold DB: A3N029

Antitoxin

• AlphaFold DB: A3N030