Detailed information of TA system TA110444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2084378..2085093
• Replicon: chromosome
• Accession: NZ_CP031866
• Organism: Actinobacillus pleuropneumoniae serovar 8 str. 405

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1101_RS09970
• Protein ID: WP_052245676.1
• Coordinates: 2084378..2084743 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: D1101_RS09975
• Protein ID: WP_005599447.1
• Coordinates: 2084806..2085093 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1101_RS09955 (D1101_10020)	2079963..2081669	-	1707	WP_005609259.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
D1101_RS09960 (D1101_10025)	2081765..2082046	-	282	WP_012263402.1	DNA-directed RNA polymerase subunit omega	-
D1101_RS09965 (D1101_10030)	2082173..2084254	-	2082	WP_039709407.1	ATP-dependent DNA helicase RecG	-
D1101_RS09970 (D1101_10035)	2084378..2084743	-	366	WP_052245676.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1101_RS09975 (D1101_10040)	2084806..2085093	-	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D1101_RS09980 (D1101_10045)	2085299..2087014	+	1716	WP_039709409.1	proline--tRNA ligase	-
D1101_RS09985 (D1101_10050)	2087080..2087268	-	189	WP_005602684.1	hypothetical protein	-
D1101_RS09990 (D1101_10055)	2087271..2087852	-	582	WP_005606496.1	sigma-70 family RNA polymerase sigma factor	-
D1101_RS09995 (D1101_10060)	2087941..2088897	+	957	WP_005619040.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
D1101_RS10000 (D1101_10065)	2088897..2089490	+	594	WP_005599453.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14015.04 Da
• Isoelectric Point: 7.9017

>T110444 WP_052245676.1 NZ_CP031866:c2084743-2084378 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
IDTNVRQWLETIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHDVLSVYQAKSFSINNEIALLASEYHIPNK
MDLNDAYIAATAKYHNLVLVTRNLKDFNRCDIRLFNPFEPN

Nucleotide

• Length: 366 bp

>T110444 NZ_CP031866:c2084743-2084378 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
ATAGATACCAATGTACGACAATGGTTAGAAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAAT
AAAAACGGGGATTTTACTTAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATGATGTAT
TAAGCGTTTATCAAGCTAAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAA
ATGGATTTAAACGATGCTTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTT
CAACCGTTGCGATATACGTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT110444 WP_005599447.1 NZ_CP031866:c2085093-2084806 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT110444 NZ_CP031866:c2085093-2084806 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5