Detailed information of TA system TA110443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1988652..1989280
• Replicon: chromosome
• Accession: NZ_CP031866
• Organism: Actinobacillus pleuropneumoniae serovar 8 str. 405

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1101_RS09390
• Protein ID: WP_005609156.1
• Coordinates: 1988882..1989280 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: D1101_RS09385
• Protein ID: WP_039709385.1
• Coordinates: 1988652..1988882 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1101_RS09375 (D1101_09440)	1986501..1988132	+	1632	WP_005613299.1	hypothetical protein	-
D1101_RS09380	1988404..1988544	-	141	WP_162835170.1	hypothetical protein	-
D1101_RS09385 (D1101_09445)	1988652..1988882	+	231	WP_039709385.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D1101_RS09390 (D1101_09450)	1988882..1989280	+	399	WP_005609156.1	tRNA(fMet)-specific endonuclease VapC	Toxin
D1101_RS09395 (D1101_09455)	1989415..1989831	-	417	WP_005609158.1	DUF417 family protein	-
D1101_RS09400 (D1101_09460)	1990078..1990728	+	651	WP_039709386.1	DUF2202 domain-containing protein	-
D1101_RS09405 (D1101_09465)	1990803..1991462	-	660	WP_039709387.1	hypothetical protein	-
D1101_RS09410	1991708..1991869	-	162	WP_160260668.1	hypothetical protein	-
D1101_RS09415 (D1101_09470)	1991862..1992533	-	672	WP_039709388.1	hypothetical protein	-
D1101_RS09420 (D1101_09475)	1992605..1993927	-	1323	WP_039709389.1	HslU--HslV peptidase ATPase subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1973170..2005099	31929

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15066.37 Da
• Isoelectric Point: 7.3824

>T110443 WP_005609156.1 NZ_CP031866:1988882-1989280 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
MLTYMLDTNIAIYVIKRRPIEVLDKFNLNSTRLCVSSITAAELYYGAEKSQFPERNMAVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKCGKILGENDIHIAAHARSEGLVLVTNNLREFERVEGLRLDNWV

Nucleotide

• Length: 399 bp

>T110443 NZ_CP031866:1988882-1989280 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
ATGCTAACTTATATGCTTGATACTAACATTGCGATTTATGTGATTAAACGCAGACCTATTGAAGTGCTGGATAAGTTTAA
TTTAAATTCTACTCGTCTTTGTGTAAGTAGTATCACTGCAGCGGAGTTGTATTATGGTGCAGAGAAAAGCCAGTTTCCGG
AACGAAATATGGCAGTAATTGAGGATTTTCTTTCTCGTTTAACGATTCTTGATTATACGCATAAGGCGGCTACTCACTTT
GGCAATATTAAAGCACATTTATCTAAGTGCGGTAAGATTCTCGGCGAGAACGATATTCATATTGCCGCGCATGCAAGAAG
TGAAGGACTCGTATTAGTAACCAATAACTTACGTGAATTTGAACGTGTAGAAGGATTGCGATTAGATAATTGGGTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8581.65 Da
• Isoelectric Point: 4.5203

>AT110443 WP_039709385.1 NZ_CP031866:1988652-1988882 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLGSATVSDDFMTEREISTQAEREDL

Nucleotide

• Length: 231 bp

>AT110443 NZ_CP031866:1988652-1988882 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
ATGGAAGCATCAGTATTTATGACAAATCGCAGTCAAGCGGTACGATTGCCGGCAGAAGTACGTTTTGCAAGTGATGTAAA
AAAACTTTCTGTAAGAGTGGTAGGTAATGAACGTATTTTGGCACCGATTAACCAAAGTTGGGATAGCTTCTTTCTGGGGT
CGGCAACGGTAAGCGATGATTTCATGACAGAGCGTGAAATCAGCACTCAAGCCGAACGTGAGGATCTATAA