Detailed information of TA system TA110442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1771080..1771811
• Replicon: chromosome
• Accession: NZ_CP031866
• Organism: Actinobacillus pleuropneumoniae serovar 8 str. 405

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: D1101_RS08300
• Protein ID: WP_005598840.1
• Coordinates: 1771080..1771547 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: D1101_RS08305
• Protein ID: WP_005619269.1
• Coordinates: 1771551..1771811 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1101_RS08275 (D1101_08330)	1766440..1766811	-	372	WP_005598830.1	CrcB family protein	-
D1101_RS08280 (D1101_08335)	1766814..1767878	-	1065	WP_039709309.1	MFS transporter	-
D1101_RS08285 (D1101_08340)	1767973..1769082	+	1110	WP_039709310.1	anhydro-N-acetylmuramic acid kinase	-
D1101_RS08290 (D1101_08345)	1769135..1770049	+	915	WP_005598836.1	N-acetylmuramic acid 6-phosphate etherase	-
D1101_RS08295 (D1101_08350)	1770116..1770997	-	882	WP_005598838.1	50S ribosomal protein L11 methyltransferase	-
D1101_RS08300 (D1101_08355)	1771080..1771547	-	468	WP_005598840.1	GNAT family N-acetyltransferase	Toxin
D1101_RS08305 (D1101_08360)	1771551..1771811	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
D1101_RS08310 (D1101_08365)	1771932..1773392	-	1461	WP_039709592.1	metalloprotease TldD	-
D1101_RS08315 (D1101_08370)	1773522..1774781	+	1260	WP_039709311.1	tRNA lysidine(34) synthetase TilS	-
D1101_RS08320 (D1101_08375)	1774808..1775098	-	291	WP_005602215.1	DUF5389 family protein	-
D1101_RS08325 (D1101_08380)	1775100..1775993	-	894	WP_005598850.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17206.22 Da
• Isoelectric Point: 8.9312

>T110442 WP_005598840.1 NZ_CP031866:c1771547-1771080 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVCDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGIKALLVHALNPQAKQFYLKYGFSCSPIDEMVLMLKL

Nucleotide

• Length: 468 bp

>T110442 NZ_CP031866:c1771547-1771080 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGTGTGATGAAAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGCCATCAGTTTGTTGCAGGTGCCTTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGTTCGGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTGGCGAACCAAATCGGGATAAAAGCATTGCTTGTTCATGCGTTAAACCCGCAAGCAAAAC
AATTTTATTTAAAGTATGGTTTTTCATGTTCACCCATTGATGAGATGGTATTAATGTTAAAACTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT110442 WP_005619269.1 NZ_CP031866:c1771811-1771551 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT110442 NZ_CP031866:c1771811-1771551 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGTATTTTAGGTAA
GACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACTGTTTTCCAATTAA
ATGAGCAGGCTTTTGAGCAATTTAACCGATTACTAGAACAACCGACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Antitoxin

• AlphaFold DB: A3N2I7