Detailed information of TA system TA110440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 1471120..1471628
• Replicon: chromosome
• Accession: NZ_CP031866
• Organism: Actinobacillus pleuropneumoniae serovar 8 str. 405

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B0BQK9
• Locus tag: D1101_RS06800
• Protein ID: WP_005605064.1
• Coordinates: 1471368..1471628 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B0BQK8
• Locus tag: D1101_RS06795
• Protein ID: WP_005598318.1
• Coordinates: 1471120..1471371 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1101_RS06755 (D1101_06825)	1466655..1467203	-	549	WP_218633997.1	type IV pilus biogenesis/stability protein PilW	-
D1101_RS06760 (D1101_06830)	1467257..1468438	-	1182	WP_005605050.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
D1101_RS06785 (D1101_06855)	1469343..1470782	+	1440	WP_005608501.1	glutamate--tRNA ligase	-
D1101_RS06795 (D1101_06865)	1471120..1471371	+	252	WP_005598318.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
D1101_RS06800 (D1101_06870)	1471368..1471628	+	261	WP_005605064.1	Txe/YoeB family addiction module toxin	Toxin
D1101_RS06805 (D1101_06875)	1471787..1471954	-	168	WP_005598320.1	Trm112 family protein	-
D1101_RS06810 (D1101_06880)	1471956..1472936	-	981	WP_005608502.1	tetraacyldisaccharide 4'-kinase	-
D1101_RS06815 (D1101_06885)	1473030..1474289	-	1260	WP_005598324.1	ATP-dependent protease ATP-binding subunit ClpX	-
D1101_RS06820 (D1101_06890)	1474289..1474879	-	591	WP_005598326.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
D1101_RS06825 (D1101_06895)	1475001..1475393	+	393	WP_005620423.1	SufE family protein	-
D1101_RS06840 (D1101_06910)	1475764..1476525	-	762	WP_039709246.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10423.99 Da
• Isoelectric Point: 8.0109

>T110440 WP_005605064.1 NZ_CP031866:1471368-1471628 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKLEPLKFNLSGFWSRRINEEHRLIYSVEDEAILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T110440 NZ_CP031866:1471368-1471628 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9362.66 Da
• Isoelectric Point: 4.5659

>AT110440 WP_005598318.1 NZ_CP031866:1471120-1471371 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYCSLEETAYLMRSPTNAKRLLDSVAELRQGQGIERE
LIE

Nucleotide

• Length: 252 bp

>AT110440 NZ_CP031866:1471120-1471371 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACGATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TGATGCGTTCACCCACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Toxin

• AlphaFold DB: B0BQK9

Antitoxin

• AlphaFold DB: B0BQK8