Detailed information of TA system TA110436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1804112..1804843
• Replicon: chromosome
• Accession: NZ_CP031865
• Organism: Actinobacillus pleuropneumoniae serovar 9 str. CVJ13261

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: D1102_RS08435
• Protein ID: WP_005598840.1
• Coordinates: 1804112..1804579 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: D1102_RS08440
• Protein ID: WP_005619269.1
• Coordinates: 1804583..1804843 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1102_RS08410 (D1102_08425)	1799471..1799842	-	372	WP_005598830.1	CrcB family protein	-
D1102_RS08415 (D1102_08430)	1799845..1800909	-	1065	WP_043991897.1	MFS transporter	-
D1102_RS08420 (D1102_08435)	1801004..1802113	+	1110	WP_005598834.1	anhydro-N-acetylmuramic acid kinase	-
D1102_RS08425 (D1102_08440)	1802167..1803081	+	915	WP_005598836.1	N-acetylmuramic acid 6-phosphate etherase	-
D1102_RS08430 (D1102_08445)	1803148..1804029	-	882	WP_005598838.1	50S ribosomal protein L11 methyltransferase	-
D1102_RS08435 (D1102_08450)	1804112..1804579	-	468	WP_005598840.1	GNAT family N-acetyltransferase	Toxin
D1102_RS08440 (D1102_08455)	1804583..1804843	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
D1102_RS08445 (D1102_08460)	1804964..1806424	-	1461	WP_005598844.1	metalloprotease TldD	-
D1102_RS08450 (D1102_08465)	1806554..1807813	+	1260	WP_005598846.1	tRNA lysidine(34) synthetase TilS	-
D1102_RS08455 (D1102_08470)	1807840..1808130	-	291	WP_005598848.1	DUF5389 family protein	-
D1102_RS08460 (D1102_08475)	1808132..1809025	-	894	WP_005598850.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17206.22 Da
• Isoelectric Point: 8.9312

>T110436 WP_005598840.1 NZ_CP031865:c1804579-1804112 [Actinobacillus pleuropneumoniae serovar 9 str. CVJ13261]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVCDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGIKALLVHALNPQAKQFYLKYGFSCSPIDEMVLMLKL

Nucleotide

• Length: 468 bp

>T110436 NZ_CP031865:c1804579-1804112 [Actinobacillus pleuropneumoniae serovar 9 str. CVJ13261]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGTGTGATGAAAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGCCATCAGTTTGTTGCAGGTGCCTTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGTTCGGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTGGCGAACCAAATCGGGATAAAAGCATTGCTTGTTCATGCGTTAAACCCGCAAGCAAAAC
AATTTTATTTAAAGTATGGTTTTTCATGTTCACCCATTGATGAGATGGTATTAATGTTAAAACTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT110436 WP_005619269.1 NZ_CP031865:c1804843-1804583 [Actinobacillus pleuropneumoniae serovar 9 str. CVJ13261]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT110436 NZ_CP031865:c1804843-1804583 [Actinobacillus pleuropneumoniae serovar 9 str. CVJ13261]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGCATTTTAGGTAA
AACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACCGTTTTCCAATTAA
ATGAGCAGGCTTTCGAGCAATTTAACCGATTGCTAGAACAGCCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Antitoxin

• AlphaFold DB: A3N2I7