Detailed information of TA system TA110432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1986255..1986883
• Replicon: chromosome
• Accession: NZ_CP031864
• Organism: Actinobacillus pleuropneumoniae serovar 10 str. D13039

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1103_RS09250
• Protein ID: WP_009875032.1
• Coordinates: 1986485..1986883 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B3GYV7
• Locus tag: D1103_RS09245
• Protein ID: WP_005613300.1
• Coordinates: 1986255..1986485 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1103_RS09240 (D1103_09310)	1984102..1985733	+	1632	WP_005613299.1	hypothetical protein	-
D1103_RS09245 (D1103_09315)	1986255..1986485	+	231	WP_005613300.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D1103_RS09250 (D1103_09320)	1986485..1986883	+	399	WP_009875032.1	tRNA(fMet)-specific endonuclease VapC	Toxin
D1103_RS09255 (D1103_09325)	1987018..1987434	-	417	WP_005613302.1	DUF417 family protein	-
D1103_RS09260 (D1103_09330)	1987681..1988331	+	651	WP_005613303.1	DUF2202 domain-containing protein	-
D1103_RS09265 (D1103_09335)	1988511..1989290	-	780	WP_005613304.1	hypothetical protein	-
D1103_RS09270 (D1103_09340)	1989363..1990685	-	1323	WP_043995163.1	HslU--HslV peptidase ATPase subunit	-
D1103_RS09275 (D1103_09345)	1990831..1991352	-	522	WP_005613306.1	ATP-dependent protease subunit HslV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1971837..2001689	29852

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-53)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15105.39 Da
• Isoelectric Point: 7.4195

>T110432 WP_009875032.1 NZ_CP031864:1986485-1986883 [Actinobacillus pleuropneumoniae serovar 10 str. D13039]
MLTYMLDTNIAIYVIKRRPIEVLDKFNLNSTRLCISSITAAELYYGAEKSQFPERNMAVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKQGKIIGENDIHIAAHARSEGLVLVTNNLREFERVEGLRLDNWV

Nucleotide

• Length: 399 bp

>T110432 NZ_CP031864:1986485-1986883 [Actinobacillus pleuropneumoniae serovar 10 str. D13039]
ATGCTAACTTATATGCTTGATACTAACATTGCGATTTATGTGATTAAACGCAGACCTATTGAAGTGCTGGATAAGTTTAA
TTTAAATTCTACCCGTCTTTGTATAAGTAGTATCACTGCAGCGGAGCTGTATTATGGTGCAGAGAAAAGCCAGTTTCCGG
AACGAAATATGGCAGTAATTGAGGATTTTCTTTCTCGTTTAACGATTCTTGATTATACGCATAAGGCGGCTACTCACTTT
GGCAATATCAAAGCACATTTATCTAAGCAAGGTAAGATCATTGGCGAGAACGATATTCATATTGCCGCACATGCAAGAAG
TGAAGGACTCGTATTAGTAACCAATAACTTGCGTGAATTTGAACGTGTGGAAGGATTGCGATTAGATAATTGGGTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8625.71 Da
• Isoelectric Point: 4.5358

>AT110432 WP_005613300.1 NZ_CP031864:1986255-1986485 [Actinobacillus pleuropneumoniae serovar 10 str. D13039]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLSSATVSDDFMTEREISTQAEREEL

Nucleotide

• Length: 231 bp

>AT110432 NZ_CP031864:1986255-1986485 [Actinobacillus pleuropneumoniae serovar 10 str. D13039]
ATGGAAGCATCAGTATTTATGACAAACCGTAGTCAGGCGGTACGATTACCTGCAGAAGTGCGTTTTGCAAGTGATGTAAA
AAAACTTTCTGTAAGAGTGGTAGGCAATGAACGTATTTTGGCTCCAATTAACCAAAGTTGGGATAGCTTCTTTCTGAGTT
CGGCAACGGTAAGCGATGATTTCATGACAGAGCGTGAAATCAGCACTCAAGCCGAACGTGAGGAACTATAA

Structures

Antitoxin

• AlphaFold DB: A3N328