Detailed information of TA system TA110431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1921048..1922705
• Replicon: chromosome
• Accession: NZ_CP031864
• Organism: Actinobacillus pleuropneumoniae serovar 10 str. D13039

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: D1103_RS08970
• Protein ID: WP_005613260.1
• Coordinates: 1921374..1922705 (-)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A3N2X7
• Locus tag: D1103_RS08965
• Protein ID: WP_005613259.1
• Coordinates: 1921048..1921377 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1103_RS08940 (D1103_09010)	1917575..1918192	+	618	WP_005613254.1	dimethylsulfoxide reductase subunit B	-
D1103_RS08945 (D1103_09015)	1918194..1919027	+	834	WP_005613255.1	dimethyl sulfoxide reductase anchor subunit	-
D1103_RS08950 (D1103_09020)	1919083..1919688	+	606	WP_005613256.1	Tat proofreading chaperone DmsD	-
D1103_RS08955 (D1103_09025)	1919692..1920210	+	519	WP_005613257.1	ferredoxin-type protein NapF	-
D1103_RS08960 (D1103_09030)	1920224..1920835	-	612	WP_005613258.1	class I SAM-dependent methyltransferase	-
D1103_RS08965 (D1103_09035)	1921048..1921377	-	330	WP_005613259.1	helix-turn-helix domain-containing protein	Antitoxin
D1103_RS08970 (D1103_09040)	1921374..1922705	-	1332	WP_005613260.1	HipA domain-containing protein	Toxin
D1103_RS08975 (D1103_09050)	1923486..1924205	-	720	WP_237610741.1	DUF5677 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1920224..1933309	13085

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (164-362)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-94)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 51121.00 Da
• Isoelectric Point: 5.2253

>T110431 WP_005613260.1 NZ_CP031864:c1922705-1921374 [Actinobacillus pleuropneumoniae serovar 10 str. D13039]
MNDISVKNKKLTLQTYVDNLWRDMAELYFNAQYELESMEYLMDYAVDFFGQANARSVSINYPVNVFIDHADKLMTTLADI
IPAGASRRYWLEALDIKHLSISHQNYLLLKNGAISPIGNVRVKEAVPEIQSERYFPLNVVQNRQSDFLEYANQTGAIVGG
ATGAGGEAPKLLLKRQDKQIWIDNLQLGLDCDTAYLVKYPRGNYSELDCDILRAEYHYYHELRALGFSTIDTDLMKLEEG
ERYPSLWLPRFDMKFKDGKWERYAMESVYSMLQKYGGNLDHEQTIRNLLNVISASQFSNDFDNRHFVIEWVKRDLLNIAF
GNSDNHGRNTAFLRDDNHIWLSPIYDFAPMRADPEGIIRTTTWSKNGDTPQEFAGEYRFDKIADGLSDLIEPDILLRELR
MLATQLVGLKSRLTKRGVPKSILDFPSIGFDYLPEKLQRWGLV

Nucleotide

• Length: 1332 bp

>T110431 NZ_CP031864:c1922705-1921374 [Actinobacillus pleuropneumoniae serovar 10 str. D13039]
ATGAATGATATATCAGTCAAAAATAAAAAATTAACATTGCAGACCTATGTTGATAATCTATGGCGAGATATGGCGGAGCT
TTATTTCAATGCTCAGTATGAATTAGAGAGCATGGAATATTTAATGGACTATGCCGTCGATTTTTTTGGGCAAGCGAATG
CCAGATCCGTATCAATTAATTATCCTGTAAATGTGTTTATTGATCATGCTGATAAGCTGATGACTACACTTGCCGATATT
ATTCCTGCCGGAGCGAGTCGGCGTTATTGGCTGGAAGCTCTTGATATCAAGCACCTGTCGATTTCTCATCAAAATTACTT
ATTATTAAAAAATGGCGCAATATCCCCTATCGGCAATGTTCGAGTTAAAGAGGCTGTGCCAGAAATACAAAGTGAGCGTT
ATTTTCCATTAAATGTGGTACAAAACAGGCAGAGTGATTTTTTAGAATATGCAAATCAGACAGGAGCTATTGTAGGCGGA
GCGACCGGGGCTGGAGGAGAAGCACCAAAGCTATTACTTAAACGCCAAGATAAACAAATTTGGATTGATAATTTACAATT
AGGTCTAGATTGCGATACGGCTTATTTAGTGAAATATCCTCGTGGGAATTATTCAGAGTTGGACTGTGATATTTTAAGAG
CTGAATATCATTATTATCATGAACTGCGTGCATTAGGGTTTTCAACTATAGATACTGATCTAATGAAATTAGAAGAGGGG
GAACGTTATCCGTCTTTATGGCTACCTAGATTCGATATGAAGTTCAAAGATGGAAAATGGGAGCGATATGCTATGGAATC
GGTTTATTCTATGTTACAAAAATATGGTGGAAATCTTGATCATGAGCAGACTATTCGCAACTTACTCAATGTTATTTCAG
CTTCGCAATTTTCTAATGATTTTGATAACCGGCATTTTGTTATTGAATGGGTAAAGCGAGATTTGTTGAATATTGCTTTT
GGAAATAGTGATAATCATGGCAGAAATACCGCATTTTTACGTGATGATAATCATATTTGGCTATCCCCTATCTATGATTT
TGCTCCGATGCGGGCAGATCCGGAAGGTATTATCAGAACGACCACTTGGTCTAAAAATGGAGATACTCCTCAAGAATTTG
CCGGAGAATATCGCTTTGATAAAATAGCTGACGGTTTATCTGACCTTATTGAACCAGATATATTGTTAAGAGAATTAAGA
ATGCTTGCCACCCAATTAGTAGGTTTAAAATCTAGACTTACGAAGCGAGGTGTTCCGAAAAGCATTTTAGATTTTCCCTC
CATTGGTTTTGATTACTTACCTGAGAAGTTACAACGTTGGGGGCTGGTATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12320.45 Da
• Isoelectric Point: 10.8292

>AT110431 WP_005613259.1 NZ_CP031864:c1921377-1921048 [Actinobacillus pleuropneumoniae serovar 10 str. D13039]
MTIQHKNASPTRSKVNGIERQLIRETIIVQLLKGEISQGQALRRLRVEALGINQQDYLKLAKVSRQTLSNIENDKGNYSI
ETINQVFKPMGLKLGLMPISKDLMASFLK

Nucleotide

• Length: 330 bp

>AT110431 NZ_CP031864:c1921377-1921048 [Actinobacillus pleuropneumoniae serovar 10 str. D13039]
ATGACGATTCAACATAAAAATGCCTCACCGACACGCTCTAAAGTGAATGGCATTGAACGACAATTAATCCGTGAAACGAT
TATAGTACAGCTCTTAAAAGGTGAGATTAGCCAAGGGCAAGCACTGCGTCGATTAAGAGTGGAAGCATTGGGAATAAACC
AGCAGGATTATCTGAAATTAGCGAAAGTTTCTCGGCAGACACTTTCTAATATCGAAAATGATAAAGGAAACTATAGTATA
GAGACAATTAATCAGGTGTTTAAACCGATGGGACTAAAGCTAGGTCTTATGCCGATTTCAAAAGACTTGATGGCTTCCTT
TCTTAAATAA

Structures

Antitoxin

• AlphaFold DB: A3N2X7