Detailed information of TA system TA110428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 1482002..1482510
• Replicon: chromosome
• Accession: NZ_CP031864
• Organism: Actinobacillus pleuropneumoniae serovar 10 str. D13039

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B0BQK9
• Locus tag: D1103_RS06740
• Protein ID: WP_005605064.1
• Coordinates: 1482250..1482510 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B0BQK8
• Locus tag: D1103_RS06735
• Protein ID: WP_005598318.1
• Coordinates: 1482002..1482253 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1103_RS06710 (D1103_06780)	1477449..1478084	+	636	WP_005612740.1	ash family protein	-
D1103_RS06715 (D1103_06785)	1478077..1478418	+	342	WP_043995129.1	hypothetical protein	-
D1103_RS06720 (D1103_06790)	1478411..1478614	+	204	WP_005612743.1	hypothetical protein	-
D1103_RS06725 (D1103_06795)	1478607..1478831	+	225	WP_014991326.1	hypothetical protein	-
D1103_RS06730 (D1103_06800)	1478809..1480980	+	2172	WP_005612747.1	DUF927 domain-containing protein	-
D1103_RS06735 (D1103_06805)	1482002..1482253	+	252	WP_005598318.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
D1103_RS06740 (D1103_06810)	1482250..1482510	+	261	WP_005605064.1	Txe/YoeB family addiction module toxin	Toxin
D1103_RS06745 (D1103_06815)	1482669..1482836	-	168	WP_005598320.1	Trm112 family protein	-
D1103_RS06750 (D1103_06820)	1482838..1483818	-	981	WP_005608502.1	tetraacyldisaccharide 4'-kinase	-
D1103_RS06755 (D1103_06825)	1483912..1485171	-	1260	WP_005598324.1	ATP-dependent protease ATP-binding subunit ClpX	-
D1103_RS06760 (D1103_06830)	1485171..1485761	-	591	WP_005598326.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
D1103_RS06765 (D1103_06835)	1485883..1486275	+	393	WP_005620423.1	SufE family protein	-
D1103_RS06780 (D1103_06850)	1486634..1487407	-	774	WP_043995130.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10423.99 Da
• Isoelectric Point: 8.0109

>T110428 WP_005605064.1 NZ_CP031864:1482250-1482510 [Actinobacillus pleuropneumoniae serovar 10 str. D13039]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKLEPLKFNLSGFWSRRINEEHRLIYSVEDEAILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T110428 NZ_CP031864:1482250-1482510 [Actinobacillus pleuropneumoniae serovar 10 str. D13039]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9362.66 Da
• Isoelectric Point: 4.5659

>AT110428 WP_005598318.1 NZ_CP031864:1482002-1482253 [Actinobacillus pleuropneumoniae serovar 10 str. D13039]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYCSLEETAYLMRSPTNAKRLLDSVAELRQGQGIERE
LIE

Nucleotide

• Length: 252 bp

>AT110428 NZ_CP031864:1482002-1482253 [Actinobacillus pleuropneumoniae serovar 10 str. D13039]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACGATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TGATGCGTTCACCCACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Toxin

• AlphaFold DB: B0BQK9

Antitoxin

• AlphaFold DB: B0BQK8