Detailed information of TA system TA110426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2017264..2017892
• Replicon: chromosome
• Accession: NZ_CP031863
• Organism: Actinobacillus pleuropneumoniae serovar 11 str. 56153

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1104_RS09490
• Protein ID: WP_005599242.1
• Coordinates: 2017494..2017892 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A828PGB0
• Locus tag: D1104_RS09485
• Protein ID: WP_005599241.1
• Coordinates: 2017264..2017494 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1104_RS09475 (D1104_09540)	2015237..2016823	+	1587	WP_005599240.1	DUF262 domain-containing protein	-
D1104_RS09480	2017016..2017189	-	174	Protein_1828	addiction module antidote protein, HigA family	-
D1104_RS09485 (D1104_09545)	2017264..2017494	+	231	WP_005599241.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D1104_RS09490 (D1104_09550)	2017494..2017892	+	399	WP_005599242.1	tRNA(fMet)-specific endonuclease VapC	Toxin
D1104_RS09495 (D1104_09555)	2018027..2018443	-	417	WP_005599244.1	DUF417 family protein	-
D1104_RS09500 (D1104_09560)	2018680..2019870	-	1191	WP_005599245.1	pentapeptide repeat-containing protein	-
D1104_RS09505 (D1104_09565)	2019923..2021245	-	1323	WP_005620148.1	HslU--HslV peptidase ATPase subunit	-
D1104_RS09510 (D1104_09570)	2021391..2021912	-	522	WP_005599247.1	ATP-dependent protease subunit HslV	-
D1104_RS09515 (D1104_09575)	2021990..2022703	-	714	WP_005609162.1	DNA/RNA nuclease SfsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2001620..2019870	18250

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15028.31 Da
• Isoelectric Point: 7.4716

>T110426 WP_005599242.1 NZ_CP031863:2017494-2017892 [Actinobacillus pleuropneumoniae serovar 11 str. 56153]
MLTYMLDTNIAIYVIKRRPLEVLDKFNLNSTRLCVSSITAAELYYGAEKSQFPEQNMAVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKHGKIIGENDIHIAAHARSEGLVLVTNNLREFERVEGLRLANWV

Nucleotide

• Length: 399 bp

>T110426 NZ_CP031863:2017494-2017892 [Actinobacillus pleuropneumoniae serovar 11 str. 56153]
ATGCTGACTTATATGCTTGATACTAACATTGCGATTTATGTCATTAAACGCAGACCACTTGAAGTGCTGGATAAGTTTAA
TTTAAATTCCACCCGTCTTTGTGTAAGTAGTATCACTGCAGCGGAGCTGTATTATGGGGCAGAGAAAAGCCAGTTTCCGG
AACAGAATATGGCAGTAATTGAGGATTTTCTTTCTCGTTTAACGATTCTTGATTATACGCATAAGGCGGCTACTCACTTT
GGCAATATCAAAGCACATTTATCTAAGCACGGTAAGATTATCGGGGAGAACGATATTCACATTGCCGCACATGCAAGAAG
TGAAGGACTCGTATTAGTAACCAATAACTTGCGTGAATTTGAACGTGTGGAAGGATTACGATTAGCTAATTGGGTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8561.71 Da
• Isoelectric Point: 4.7063

>AT110426 WP_005599241.1 NZ_CP031863:2017264-2017494 [Actinobacillus pleuropneumoniae serovar 11 str. 56153]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLGSATVSDDFMTEREISIQPEREGL

Nucleotide

• Length: 231 bp

>AT110426 NZ_CP031863:2017264-2017494 [Actinobacillus pleuropneumoniae serovar 11 str. 56153]
ATGGAAGCATCGGTATTTATGACAAACCGTAGTCAAGCGGTACGATTGCCGGCAGAAGTACGCTTTGCAAGTGATGTAAA
AAAACTTTCTGTAAGAGTAGTCGGTAATGAGCGTATTTTGGCTCCGATTAACCAAAGTTGGGATAGCTTCTTTCTGGGGT
CGGCAACGGTAAGCGATGATTTCATGACAGAGCGTGAAATCAGCATTCAGCCCGAACGTGAGGGACTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A828PGB0