Detailed information of TA system TA110425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1803813..1804544
• Replicon: chromosome
• Accession: NZ_CP031863
• Organism: Actinobacillus pleuropneumoniae serovar 11 str. 56153

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: D1104_RS08440
• Protein ID: WP_005598840.1
• Coordinates: 1803813..1804280 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: D1104_RS08445
• Protein ID: WP_005619269.1
• Coordinates: 1804284..1804544 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1104_RS08415 (D1104_08455)	1799172..1799543	-	372	WP_005598830.1	CrcB family protein	-
D1104_RS08420 (D1104_08460)	1799546..1800610	-	1065	WP_043991897.1	MFS transporter	-
D1104_RS08425 (D1104_08465)	1800705..1801814	+	1110	WP_005598834.1	anhydro-N-acetylmuramic acid kinase	-
D1104_RS08430 (D1104_08470)	1801868..1802782	+	915	WP_005598836.1	N-acetylmuramic acid 6-phosphate etherase	-
D1104_RS08435 (D1104_08475)	1802849..1803730	-	882	WP_005598838.1	50S ribosomal protein L11 methyltransferase	-
D1104_RS08440 (D1104_08480)	1803813..1804280	-	468	WP_005598840.1	GNAT family N-acetyltransferase	Toxin
D1104_RS08445 (D1104_08485)	1804284..1804544	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
D1104_RS08450 (D1104_08490)	1804665..1806125	-	1461	WP_005598844.1	metalloprotease TldD	-
D1104_RS08455 (D1104_08495)	1806255..1807514	+	1260	WP_005598846.1	tRNA lysidine(34) synthetase TilS	-
D1104_RS08460 (D1104_08500)	1807541..1807831	-	291	WP_005598848.1	DUF5389 family protein	-
D1104_RS08465 (D1104_08505)	1807833..1808726	-	894	WP_005598850.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17206.22 Da
• Isoelectric Point: 8.9312

>T110425 WP_005598840.1 NZ_CP031863:c1804280-1803813 [Actinobacillus pleuropneumoniae serovar 11 str. 56153]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVCDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGIKALLVHALNPQAKQFYLKYGFSCSPIDEMVLMLKL

Nucleotide

• Length: 468 bp

>T110425 NZ_CP031863:c1804280-1803813 [Actinobacillus pleuropneumoniae serovar 11 str. 56153]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGTGTGATGAAAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGCCATCAGTTTGTTGCAGGTGCCTTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGTTCGGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTGGCGAACCAAATCGGGATAAAAGCATTGCTTGTTCATGCGTTAAACCCGCAAGCAAAAC
AATTTTATTTAAAGTATGGTTTTTCATGTTCACCCATTGATGAGATGGTATTAATGTTAAAACTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT110425 WP_005619269.1 NZ_CP031863:c1804544-1804284 [Actinobacillus pleuropneumoniae serovar 11 str. 56153]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT110425 NZ_CP031863:c1804544-1804284 [Actinobacillus pleuropneumoniae serovar 11 str. 56153]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGCATTTTAGGTAA
AACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACCGTTTTCCAATTAA
ATGAGCAGGCTTTCGAGCAATTTAACCGATTGCTAGAACAGCCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Antitoxin

• AlphaFold DB: A3N2I7