Detailed information of TA system TA110423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 1509451..1509959
• Replicon: chromosome
• Accession: NZ_CP031863
• Organism: Actinobacillus pleuropneumoniae serovar 11 str. 56153

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B0BQK9
• Locus tag: D1104_RS06975
• Protein ID: WP_005605064.1
• Coordinates: 1509699..1509959 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: D1104_RS06970
• Protein ID: WP_005610475.1
• Coordinates: 1509451..1509702 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1104_RS06930 (D1104_06970)	1504988..1505536	-	549	WP_005598298.1	type IV pilus biogenesis/stability protein PilW	-
D1104_RS06935 (D1104_06975)	1505590..1506771	-	1182	WP_005598300.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
D1104_RS06960 (D1104_07000)	1507675..1509114	+	1440	WP_005598316.1	glutamate--tRNA ligase	-
D1104_RS06970 (D1104_07010)	1509451..1509702	+	252	WP_005610475.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
D1104_RS06975 (D1104_07015)	1509699..1509959	+	261	WP_005605064.1	Txe/YoeB family addiction module toxin	Toxin
D1104_RS06980 (D1104_07020)	1510118..1510285	-	168	WP_005598320.1	Trm112 family protein	-
D1104_RS06985 (D1104_07025)	1510287..1511267	-	981	WP_005598322.1	tetraacyldisaccharide 4'-kinase	-
D1104_RS06990 (D1104_07030)	1511361..1512620	-	1260	WP_005598324.1	ATP-dependent protease ATP-binding subunit ClpX	-
D1104_RS06995 (D1104_07035)	1512620..1513210	-	591	WP_005598326.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
D1104_RS07000 (D1104_07040)	1513332..1513724	+	393	WP_005620423.1	SufE family protein	-
D1104_RS07015 (D1104_07055)	1514083..1514856	-	774	WP_005598329.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10423.99 Da
• Isoelectric Point: 8.0109

>T110423 WP_005605064.1 NZ_CP031863:1509699-1509959 [Actinobacillus pleuropneumoniae serovar 11 str. 56153]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKLEPLKFNLSGFWSRRINEEHRLIYSVEDEAILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T110423 NZ_CP031863:1509699-1509959 [Actinobacillus pleuropneumoniae serovar 11 str. 56153]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 4.5659

>AT110423 WP_005610475.1 NZ_CP031863:1509451-1509702 [Actinobacillus pleuropneumoniae serovar 11 str. 56153]
MQAITYTEARQNLAGTMSKVAEDFEPILIIRSKGGNCVLMSYEQYCSLEETAYLMRSPTNAKRLLDSVAELRQGQGIERE
LIE

Nucleotide

• Length: 252 bp

>AT110423 NZ_CP031863:1509451-1509702 [Actinobacillus pleuropneumoniae serovar 11 str. 56153]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACGATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTATAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TGATGCGTTCACCCACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Toxin

• AlphaFold DB: B0BQK9