Detailed information of TA system TA110422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2026026..2026741
• Replicon: chromosome
• Accession: NZ_CP031862
• Organism: Actinobacillus pleuropneumoniae strain 8329

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1105_RS09505
• Protein ID: WP_052293849.1
• Coordinates: 2026026..2026391 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: D1105_RS09510
• Protein ID: WP_005599447.1
• Coordinates: 2026454..2026741 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1105_RS09490 (D1105_09580)	2021611..2023317	-	1707	WP_005616199.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
D1105_RS09495 (D1105_09585)	2023413..2023694	-	282	WP_005599441.1	DNA-directed RNA polymerase subunit omega	-
D1105_RS09500 (D1105_09590)	2023821..2025902	-	2082	WP_005616200.1	ATP-dependent DNA helicase RecG	-
D1105_RS09505 (D1105_09595)	2026026..2026391	-	366	WP_052293849.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1105_RS09510 (D1105_09600)	2026454..2026741	-	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D1105_RS09515 (D1105_09605)	2026947..2028662	+	1716	WP_005616202.1	proline--tRNA ligase	-
D1105_RS09520 (D1105_09610)	2028728..2028916	-	189	WP_005602684.1	hypothetical protein	-
D1105_RS09525 (D1105_09615)	2028919..2029500	-	582	WP_005606496.1	sigma-70 family RNA polymerase sigma factor	-
D1105_RS09530 (D1105_09620)	2029589..2030545	+	957	WP_005619040.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
D1105_RS09535 (D1105_09625)	2030545..2031138	+	594	WP_005599453.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14014.06 Da
• Isoelectric Point: 8.4956

>T110422 WP_052293849.1 NZ_CP031862:c2026391-2026026 [Actinobacillus pleuropneumoniae]
IDTNVRQWLETIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQAKSFSINNEIALLASEYHIPNK
MDLNDAYIAATAKYHNLVLVTRNLKDFNRCDIRLFNPFEPN

Nucleotide

• Length: 366 bp

>T110422 NZ_CP031862:c2026391-2026026 [Actinobacillus pleuropneumoniae]
ATAGATACCAATGTACGACAATGGTTAGAAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAAT
AAAAACGGGGATTTTACTTAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTAT
TAAGCGTTTATCAAGCTAAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAA
ATGGATTTAAACGATGCTTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTT
CAACCGTTGCGATATACGTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT110422 WP_005599447.1 NZ_CP031862:c2026741-2026454 [Actinobacillus pleuropneumoniae]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT110422 NZ_CP031862:c2026741-2026454 [Actinobacillus pleuropneumoniae]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5