Detailed information of TA system TA110421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1932357..1932985
• Replicon: chromosome
• Accession: NZ_CP031862
• Organism: Actinobacillus pleuropneumoniae strain 8329

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1105_RS08935
• Protein ID: WP_043995369.1
• Coordinates: 1932587..1932985 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BSF9
• Locus tag: D1105_RS08930
• Protein ID: WP_005616131.1
• Coordinates: 1932357..1932587 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1105_RS08920 (D1105_09005)	1929664..1931214	+	1551	WP_005616128.1	class I SAM-dependent methyltransferase	-
D1105_RS08925 (D1105_09010)	1931211..1931993	+	783	WP_005602534.1	HincII family type II restriction endonuclease	-
D1105_RS08930 (D1105_09015)	1932357..1932587	+	231	WP_005616131.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D1105_RS08935 (D1105_09020)	1932587..1932985	+	399	WP_043995369.1	tRNA(fMet)-specific endonuclease VapC	Toxin
D1105_RS08940 (D1105_09025)	1933120..1933536	-	417	WP_005599244.1	DUF417 family protein	-
D1105_RS08945 (D1105_09030)	1933782..1934432	+	651	WP_005616137.1	DUF2202 domain-containing protein	-
D1105_RS08950 (D1105_09035)	1934505..1935164	-	660	WP_005605767.1	hypothetical protein	-
D1105_RS08955 (D1105_09040)	1935478..1936800	-	1323	WP_005620148.1	HslU--HslV peptidase ATPase subunit	-
D1105_RS08960 (D1105_09045)	1936946..1937467	-	522	WP_005613306.1	ATP-dependent protease subunit HslV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1914992..1947797	32805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15033.33 Da
• Isoelectric Point: 8.0810

>T110421 WP_043995369.1 NZ_CP031862:1932587-1932985 [Actinobacillus pleuropneumoniae]
MLTYMLDTNIAIYVVKRRPIEVLDKFNLNSTRLCVSSITAAELYYGAEKSQFPERNMAVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKQGKIIGENDIHIAAHARSEGLVLVTNNLREFERVEGLRLANWV

Nucleotide

• Length: 399 bp

>T110421 NZ_CP031862:1932587-1932985 [Actinobacillus pleuropneumoniae]
ATGCTAACTTATATGCTTGATACTAACATTGCGATTTATGTGGTTAAACGCAGACCTATTGAAGTGCTGGATAAGTTTAA
TTTAAATTCTACCCGTCTTTGTGTAAGTAGTATCACTGCAGCGGAGCTGTATTATGGTGCAGAGAAAAGCCAGTTTCCGG
AACGAAATATGGCAGTAATTGAGGATTTTCTTTCTCGTTTAACGATTCTTGATTATACGCATAAGGCGGCTACTCACTTT
GGCAATATCAAAGCACATTTATCTAAGCAAGGTAAGATCATTGGCGAGAACGATATTCATATTGCCGCGCATGCAAGAAG
TGAAGGACTCGTATTAGTAACCAATAACTTACGTGAATTTGAACGTGTGGAAGGATTACGATTAGCTAATTGGGTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8595.68 Da
• Isoelectric Point: 4.5358

>AT110421 WP_005616131.1 NZ_CP031862:1932357-1932587 [Actinobacillus pleuropneumoniae]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLGSATVSDDFMTEREISTQAEREEL

Nucleotide

• Length: 231 bp

>AT110421 NZ_CP031862:1932357-1932587 [Actinobacillus pleuropneumoniae]
ATGGAAGCATCAGTATTTATGACAAATCGTAGCCAAGCGGTACGATTACCTGCAGAAGTGCGTTTTGCAAGTGATGTAAA
AAAACTTTCTGTAAGAGTGGTAGGCAATGAACGTATTTTGGCTCCAATTAACCAAAGTTGGGATAGCTTCTTTCTGGGGT
CGGCAACGGTAAGCGATGATTTCATGACAGAGCGTGAAATCAGCACTCAAGCCGAACGTGAGGAACTATAA

Structures

Antitoxin

• AlphaFold DB: B0BSF9