Detailed information of TA system TA110420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1721146..1721877
• Replicon: chromosome
• Accession: NZ_CP031862
• Organism: Actinobacillus pleuropneumoniae strain 8329

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: D1105_RS07895
• Protein ID: WP_005598840.1
• Coordinates: 1721146..1721613 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: D1105_RS07900
• Protein ID: WP_005619269.1
• Coordinates: 1721617..1721877 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1105_RS07870 (D1105_07935)	1716506..1716877	-	372	WP_005598830.1	CrcB family protein	-
D1105_RS07875 (D1105_07940)	1716880..1717944	-	1065	WP_043995354.1	MFS transporter	-
D1105_RS07880 (D1105_07945)	1718039..1719148	+	1110	WP_005615922.1	anhydro-N-acetylmuramic acid kinase	-
D1105_RS07885 (D1105_07950)	1719201..1720115	+	915	WP_005615923.1	N-acetylmuramic acid 6-phosphate etherase	-
D1105_RS07890 (D1105_07955)	1720182..1721063	-	882	WP_005615925.1	50S ribosomal protein L11 methyltransferase	-
D1105_RS07895 (D1105_07960)	1721146..1721613	-	468	WP_005598840.1	GNAT family N-acetyltransferase	Toxin
D1105_RS07900 (D1105_07965)	1721617..1721877	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
D1105_RS07905 (D1105_07970)	1721998..1723458	-	1461	WP_005598844.1	metalloprotease TldD	-
D1105_RS07910 (D1105_07975)	1723588..1724847	+	1260	WP_005615928.1	tRNA lysidine(34) synthetase TilS	-
D1105_RS07915 (D1105_07980)	1724874..1725164	-	291	WP_005602215.1	DUF5389 family protein	-
D1105_RS07920 (D1105_07985)	1725166..1726059	-	894	WP_005598850.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17206.22 Da
• Isoelectric Point: 8.9312

>T110420 WP_005598840.1 NZ_CP031862:c1721613-1721146 [Actinobacillus pleuropneumoniae]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVCDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGIKALLVHALNPQAKQFYLKYGFSCSPIDEMVLMLKL

Nucleotide

• Length: 468 bp

>T110420 NZ_CP031862:c1721613-1721146 [Actinobacillus pleuropneumoniae]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGTGTGATGAAAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGCCATCAGTTTGTTGCAGGTGCCTTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGTTCGGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTGGCGAACCAAATCGGGATAAAAGCATTGCTTGTTCATGCGTTAAACCCGCAAGCAAAAC
AATTTTATTTAAAGTATGGTTTTTCATGTTCACCCATTGATGAGATGGTATTAATGTTAAAACTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT110420 WP_005619269.1 NZ_CP031862:c1721877-1721617 [Actinobacillus pleuropneumoniae]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT110420 NZ_CP031862:c1721877-1721617 [Actinobacillus pleuropneumoniae]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGCATTTTAGGTAA
AACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACCGTTTTCCAATTAA
ATGAGCAGGCTTTCGAGCAATTTAACCGATTGCTAGAACAGCCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Antitoxin

• AlphaFold DB: A3N2I7