Detailed information of TA system TA110418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 1422556..1423064
• Replicon: chromosome
• Accession: NZ_CP031862
• Organism: Actinobacillus pleuropneumoniae strain 8329

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B0BQK9
• Locus tag: D1105_RS06400
• Protein ID: WP_005605064.1
• Coordinates: 1422804..1423064 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B0BQK8
• Locus tag: D1105_RS06395
• Protein ID: WP_005598318.1
• Coordinates: 1422556..1422807 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1105_RS06355 (D1105_06405)	1418092..1418640	-	549	WP_005615686.1	type IV pilus biogenesis/stability protein PilW	-
D1105_RS06360 (D1105_06410)	1418694..1419875	-	1182	WP_005608495.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
D1105_RS06385 (D1105_06435)	1420779..1422218	+	1440	WP_005615688.1	glutamate--tRNA ligase	-
D1105_RS06395 (D1105_06445)	1422556..1422807	+	252	WP_005598318.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
D1105_RS06400 (D1105_06450)	1422804..1423064	+	261	WP_005605064.1	Txe/YoeB family addiction module toxin	Toxin
D1105_RS06405 (D1105_06455)	1423223..1423390	-	168	WP_005598320.1	Trm112 family protein	-
D1105_RS06410 (D1105_06460)	1423392..1424372	-	981	WP_005608502.1	tetraacyldisaccharide 4'-kinase	-
D1105_RS06415 (D1105_06465)	1424466..1425725	-	1260	WP_005615690.1	ATP-dependent protease ATP-binding subunit ClpX	-
D1105_RS06420 (D1105_06470)	1425725..1426315	-	591	WP_005598326.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
D1105_RS06425 (D1105_06475)	1426437..1426829	+	393	WP_005620423.1	SufE family protein	-
D1105_RS06440 (D1105_06490)	1427188..1427961	-	774	WP_043995337.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10423.99 Da
• Isoelectric Point: 8.0109

>T110418 WP_005605064.1 NZ_CP031862:1422804-1423064 [Actinobacillus pleuropneumoniae]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKLEPLKFNLSGFWSRRINEEHRLIYSVEDEAILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T110418 NZ_CP031862:1422804-1423064 [Actinobacillus pleuropneumoniae]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9362.66 Da
• Isoelectric Point: 4.5659

>AT110418 WP_005598318.1 NZ_CP031862:1422556-1422807 [Actinobacillus pleuropneumoniae]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYCSLEETAYLMRSPTNAKRLLDSVAELRQGQGIERE
LIE

Nucleotide

• Length: 252 bp

>AT110418 NZ_CP031862:1422556-1422807 [Actinobacillus pleuropneumoniae]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACGATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TGATGCGTTCACCCACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Toxin

• AlphaFold DB: B0BQK9

Antitoxin

• AlphaFold DB: B0BQK8