Detailed information of TA system TA110417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 724418..725068
• Replicon: chromosome
• Accession: NZ_CP031862
• Organism: Actinobacillus pleuropneumoniae strain 8329

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B0BNU3
• Locus tag: D1105_RS03255
• Protein ID: WP_005614926.1
• Coordinates: 724418..724807 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BNU4
• Locus tag: D1105_RS03260
• Protein ID: WP_005604117.1
• Coordinates: 724808..725068 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1105_RS03240 (D1105_03240)	720215..721255	+	1041	WP_237611566.1	IS481 family transposase	-
D1105_RS03245 (D1105_03245)	721364..722650	-	1287	WP_005614923.1	Xaa-Pro aminopeptidase	-
D1105_RS03250 (D1105_03250)	723059..724282	-	1224	WP_005614925.1	ATP-binding protein	-
D1105_RS03255 (D1105_03255)	724418..724807	-	390	WP_005614926.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1105_RS03260 (D1105_03260)	724808..725068	-	261	WP_005604117.1	hypothetical protein	Antitoxin
D1105_RS03265 (D1105_03265)	725414..726250	+	837	WP_005614928.1	DUF6216 family protein	-
D1105_RS03270 (D1105_03270)	726282..728303	-	2022	WP_005614930.1	excinuclease ABC subunit UvrB	-
D1105_RS03275 (D1105_03280)	728480..729292	+	813	WP_005607533.1	iron uptake system protein EfeO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	720215..721255	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14837.17 Da
• Isoelectric Point: 5.1295

>T110417 WP_005614926.1 NZ_CP031862:c724807-724418 [Actinobacillus pleuropneumoniae]
MYMLDTNTVSYFFRQDPTVVKKLQQLNPELICISSVTAAELFYGVKKRNNQKLTAFLNTFLSAITVMDWDYQVAEVYGQL
RAEMEREGKIMGVQDQMIGAHALATECVLVSSDKAFQFIPNLVLENWWK

Nucleotide

• Length: 390 bp

>T110417 NZ_CP031862:c724807-724418 [Actinobacillus pleuropneumoniae]
ATGTATATGCTAGATACTAATACGGTGAGTTATTTCTTTAGGCAAGATCCTACTGTGGTTAAGAAACTACAACAACTTAA
TCCGGAATTGATTTGTATTTCATCGGTCACGGCTGCAGAGTTGTTTTATGGCGTAAAAAAACGTAATAATCAGAAACTTA
CTGCATTTTTAAATACTTTTTTATCTGCGATTACGGTGATGGATTGGGATTATCAAGTGGCGGAAGTTTATGGTCAGCTG
CGAGCAGAGATGGAACGAGAAGGCAAAATCATGGGCGTTCAAGATCAAATGATTGGAGCTCATGCCCTAGCAACAGAATG
TGTTCTTGTTTCCAGCGATAAAGCCTTTCAATTTATCCCTAATCTCGTATTAGAAAATTGGTGGAAGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10289.63 Da
• Isoelectric Point: 5.1867

>AT110417 WP_005604117.1 NZ_CP031862:c725068-724808 [Actinobacillus pleuropneumoniae]
MEKVARLFRNGRNQAVRLPVEFEFNAKQVYVFKEANGDITLSTRSRNQQNWQKVFELLPMLECDEQFLSEQERKQEMATR
DPFEGF

Nucleotide

• Length: 261 bp

>AT110417 NZ_CP031862:c725068-724808 [Actinobacillus pleuropneumoniae]
ATGGAAAAGGTCGCACGTTTATTTAGAAACGGACGTAATCAAGCCGTACGATTACCGGTTGAATTTGAGTTTAATGCGAA
GCAAGTTTATGTGTTTAAAGAAGCAAACGGCGATATTACGCTTTCGACACGTTCTCGCAATCAGCAAAATTGGCAGAAAG
TCTTTGAGTTATTACCAATGCTTGAATGTGATGAACAGTTTTTATCGGAACAAGAACGCAAGCAAGAAATGGCAACTCGA
GATCCGTTCGAGGGCTTTTAA

Structures

Toxin

• AlphaFold DB: A3N029

Antitoxin

• AlphaFold DB: A3N030