Detailed information of TA system TA110414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1915599..1917256
• Replicon: chromosome
• Accession: NZ_CP031861
• Organism: Actinobacillus pleuropneumoniae strain N 273

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: D1106_RS09010
• Protein ID: WP_237593868.1
• Coordinates: 1915925..1917256 (-)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A3N2X7
• Locus tag: D1106_RS09005
• Protein ID: WP_005613259.1
• Coordinates: 1915599..1915928 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1106_RS08980 (D1106_09020)	1912125..1912742	+	618	WP_005613254.1	dimethylsulfoxide reductase subunit B	-
D1106_RS08985 (D1106_09025)	1912744..1913577	+	834	WP_237593864.1	dimethyl sulfoxide reductase anchor subunit family protein	-
D1106_RS08990 (D1106_09030)	1913633..1914238	+	606	WP_237593865.1	Tat proofreading chaperone DmsD	-
D1106_RS08995 (D1106_09035)	1914242..1914760	+	519	WP_237593866.1	ferredoxin-type protein NapF	-
D1106_RS09000 (D1106_09040)	1914757..1915386	-	630	WP_237593867.1	class I SAM-dependent methyltransferase	-
D1106_RS09005 (D1106_09045)	1915599..1915928	-	330	WP_005613259.1	helix-turn-helix domain-containing protein	Antitoxin
D1106_RS09010 (D1106_09050)	1915925..1917256	-	1332	WP_237593868.1	HipA domain-containing protein	Toxin
D1106_RS09015 (D1106_09060)	1918037..1918756	-	720	WP_237593869.1	DUF5677 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1915599..1927860	12261

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (164-364)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-94)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 51094.02 Da
• Isoelectric Point: 5.2313

>T110414 WP_237593868.1 NZ_CP031861:c1917256-1915925 [Actinobacillus pleuropneumoniae]
MNDISVKNKKLTLQTYVDNLWRDMAELYFNAQYELESMEYLMDYAVDFFGQANARSVSINYPVNVFIDHADKLMTTLADI
IPAGASRRYWLEALDIKHLSISHQNYLLLKNGAISPIGNVRVKEAVPEIQSERYFPLNVVQNRQSDFLEYANQTGAIVGG
ATGAGGEAPKLLLKRQDKQVWIDNLQLGLDCDTAYLVKYPRGNYSELDCDILRAEYHYYHELRALGFSTIDTDLMKLEEG
ERYPSLWLPRFDMKFKDGKWERYAMESVYSMLQKYGGNLDHEQTIRNLLNVISASQFSNDFDNRHFVIEWVKRDLLNIAF
GNSDNHGRNTAFLRDDNRIWLSPIYDFAPMRADPEGIIRTTTWAKNGDTPQEFAGEYRFDKIADGLSDLIEPDILLRELR
MLATQLVGLKSRLTKRGVPKSILDFPAIGFDYLPEKLQRWGLV

Nucleotide

• Length: 1332 bp

>T110414 NZ_CP031861:c1917256-1915925 [Actinobacillus pleuropneumoniae]
ATGAATGATATATCAGTCAAAAATAAAAAATTAACATTGCAGACCTATGTTGATAATCTATGGCGAGATATGGCGGAGCT
TTATTTCAATGCTCAGTATGAATTAGAGAGCATGGAATATTTAATGGACTATGCCGTCGATTTTTTTGGGCAAGCGAATG
CCAGATCCGTATCAATTAATTATCCTGTAAATGTGTTTATTGATCATGCTGATAAGCTGATGACTACACTTGCCGATATT
ATTCCTGCCGGAGCGAGTCGGCGTTATTGGCTGGAAGCTCTTGATATCAAGCACCTATCTATTTCTCATCAAAATTACTT
ATTGCTAAAAAATGGCGCAATATCCCCTATCGGCAATGTCCGAGTTAAAGAGGCTGTGCCAGAAATACAAAGTGAGCGTT
ATTTTCCATTAAATGTGGTACAAAACAGGCAGAGTGATTTTTTAGAATATGCAAATCAGACAGGAGCTATTGTAGGCGGA
GCGACCGGGGCTGGGGGAGAAGCACCAAAGCTATTACTTAAACGCCAAGATAAACAAGTTTGGATTGATAATTTACAATT
AGGTCTAGATTGCGATACGGCTTATTTAGTGAAATATCCTCGTGGGAATTATTCAGAGTTGGACTGTGATATTTTAAGAG
CTGAATATCATTATTATCATGAACTGCGTGCATTAGGGTTTTCAACTATAGATACTGATCTAATGAAATTAGAAGAGGGG
GAACGTTATCCGTCTTTATGGCTACCTAGATTCGATATGAAGTTCAAAGATGGAAAATGGGAGCGATATGCTATGGAATC
GGTTTATTCTATGTTACAAAAATATGGTGGAAATCTTGATCATGAGCAGACTATTCGCAACTTACTCAATGTTATTTCAG
CTTCGCAATTTTCTAATGATTTTGATAACCGGCATTTTGTTATTGAATGGGTAAAGCGAGATTTGTTGAATATTGCTTTT
GGAAATAGTGATAATCATGGCAGAAATACCGCATTTTTACGTGATGATAATCGTATTTGGCTATCCCCTATCTATGATTT
TGCTCCGATGCGGGCAGATCCGGAAGGTATTATCAGAACGACTACTTGGGCTAAAAATGGAGATACTCCTCAAGAATTCG
CTGGAGAATATCGCTTTGATAAAATAGCTGACGGTTTATCTGACCTTATTGAACCAGATATATTGTTAAGAGAATTAAGA
ATGCTTGCCACCCAATTAGTAGGTTTAAAATCTAGACTTACGAAGCGAGGTGTTCCGAAAAGCATTTTAGATTTTCCCGC
CATTGGTTTTGATTACTTACCTGAGAAGTTACAACGTTGGGGGCTGGTATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12320.45 Da
• Isoelectric Point: 10.8292

>AT110414 WP_005613259.1 NZ_CP031861:c1915928-1915599 [Actinobacillus pleuropneumoniae]
MTIQHKNASPTRSKVNGIERQLIRETIIVQLLKGEISQGQALRRLRVEALGINQQDYLKLAKVSRQTLSNIENDKGNYSI
ETINQVFKPMGLKLGLMPISKDLMASFLK

Nucleotide

• Length: 330 bp

>AT110414 NZ_CP031861:c1915928-1915599 [Actinobacillus pleuropneumoniae]
ATGACGATTCAACATAAAAATGCCTCACCAACGCGCTCTAAAGTGAATGGCATTGAACGACAATTAATCCGTGAAACGAT
TATAGTACAGCTCTTAAAAGGTGAGATTAGCCAAGGGCAAGCACTGCGTCGATTAAGAGTGGAAGCATTGGGAATAAACC
AGCAGGATTATCTGAAATTAGCGAAAGTTTCTCGGCAGACACTTTCTAATATCGAAAATGATAAAGGAAACTATAGTATA
GAGACAATTAATCAGGTGTTTAAACCGATGGGACTAAAGCTAGGTCTTATGCCGATTTCAAAAGACTTGATGGCTTCCTT
TCTTAAATAA

Structures

Antitoxin

• AlphaFold DB: A3N2X7