Detailed information of TA system TA110412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 1549442..1550087
• Replicon: chromosome
• Accession: NZ_CP031861
• Organism: Actinobacillus pleuropneumoniae strain N 273

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: B0BQU7
• Locus tag: D1106_RS07160
• Protein ID: WP_005619674.1
• Coordinates: 1549716..1550087 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: D1106_RS07155
• Protein ID: WP_005598477.1
• Coordinates: 1549442..1549735 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1106_RS07125 (D1106_07175)	1544774..1545457	-	684	WP_005605188.1	arginine ABC transporter permease ArtM	-
D1106_RS07130 (D1106_07180)	1545457..1546128	-	672	WP_005608588.1	arginine ABC transporter permease ArtQ	-
D1106_RS07135 (D1106_07185)	1546134..1546853	-	720	WP_005619670.1	transporter substrate-binding domain-containing protein	-
D1106_RS07140 (D1106_07190)	1546873..1547607	-	735	WP_005612838.1	arginine ABC transporter ATP-binding protein ArtP	-
D1106_RS07145 (D1106_07195)	1547832..1548329	-	498	WP_005598474.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
D1106_RS07150 (D1106_07200)	1548402..1549364	+	963	WP_237594331.1	calcium/sodium antiporter	-
D1106_RS07155 (D1106_07205)	1549442..1549735	-	294	WP_005598477.1	helix-turn-helix domain-containing protein	Antitoxin
D1106_RS07160 (D1106_07210)	1549716..1550087	-	372	WP_005619674.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D1106_RS07165 (D1106_07215)	1550281..1552032	+	1752	WP_237593757.1	protein-disulfide reductase DsbD	-
D1106_RS07170 (D1106_07220)	1552090..1552659	+	570	WP_005601959.1	elongation factor P hydroxylase	-
D1106_RS07175 (D1106_07225)	1552703..1553557	-	855	WP_005598486.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (32-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-85)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 15066.57 Da
• Isoelectric Point: 10.4054

>T110412 WP_005619674.1 NZ_CP031861:c1550087-1549716 [Actinobacillus pleuropneumoniae]
MYEIVFYRDKRGREPVKEFLLRLLKERQEGSRERLHKISHHLSILHLHGTRAGENYIKHLEDRIWQLRPVGDCLLFASII
RGKFVLLHYFAKQNYRIPKREIERAKVRLADLQERIKDEPYWF

Nucleotide

• Length: 372 bp

>T110412 NZ_CP031861:c1550087-1549716 [Actinobacillus pleuropneumoniae]
ATGTATGAAATAGTCTTTTATCGAGATAAGCGGGGGAGAGAACCCGTAAAAGAATTTCTGTTAAGGCTACTAAAAGAACG
GCAGGAAGGCAGTCGAGAACGATTACACAAAATCAGCCATCATTTGAGTATTTTACATTTGCACGGTACTCGAGCCGGTG
AGAATTATATTAAGCATTTGGAAGATCGTATTTGGCAGTTACGTCCGGTGGGCGATTGCCTGTTATTTGCAAGTATTATT
CGGGGAAAATTTGTGTTGTTGCATTATTTTGCCAAACAGAATTATCGAATTCCGAAACGAGAAATAGAACGTGCAAAAGT
TCGGTTGGCGGATTTACAGGAGAGAATAAAAGATGAGCCCTATTGGTTCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10935.81 Da
• Isoelectric Point: 10.3120

>AT110412 WP_005598477.1 NZ_CP031861:c1549735-1549442 [Actinobacillus pleuropneumoniae]
MSPIGSSWTSFEQEIFDESEIAETQFRIRLILEIIETRQQLGISQRKLEKLSGVKQSMIARVEKGSVNPSLATVLKLLVP
LGKTLQIVPLDKNKTVR

Nucleotide

• Length: 294 bp

>AT110412 NZ_CP031861:c1549735-1549442 [Actinobacillus pleuropneumoniae]
ATGAGCCCTATTGGTTCTAGTTGGACATCGTTTGAACAAGAAATTTTTGACGAGTCGGAAATAGCGGAAACTCAGTTTCG
CATACGACTTATTTTAGAAATCATTGAAACCCGTCAGCAGCTCGGTATTTCTCAACGAAAATTGGAGAAATTAAGCGGCG
TAAAACAATCTATGATTGCTCGGGTAGAAAAAGGCTCGGTTAATCCGTCGTTGGCGACGGTATTAAAGTTGCTTGTGCCG
TTGGGGAAAACTTTACAAATCGTACCGTTAGATAAAAACAAAACGGTACGTTAA

Structures

Toxin

• AlphaFold DB: A3N206