Detailed information of TA system TA110407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1684319..1685050
• Replicon: chromosome
• Accession: NZ_CP031860
• Organism: Actinobacillus pleuropneumoniae strain 3906

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: D1107_RS07715
• Protein ID: WP_237592683.1
• Coordinates: 1684319..1684786 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: D1107_RS07720
• Protein ID: WP_005619269.1
• Coordinates: 1684790..1685050 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1107_RS07690 (D1107_07775)	1679675..1680046	-	372	WP_005598830.1	CrcB family protein	-
D1107_RS07695 (D1107_07780)	1680049..1681113	-	1065	WP_043990971.1	MFS transporter	-
D1107_RS07700 (D1107_07785)	1681208..1682317	+	1110	WP_005605455.1	anhydro-N-acetylmuramic acid kinase	-
D1107_RS07705 (D1107_07790)	1682371..1683285	+	915	WP_237592682.1	N-acetylmuramic acid 6-phosphate etherase	-
D1107_RS07710 (D1107_07795)	1683355..1684236	-	882	WP_005608878.1	50S ribosomal protein L11 methyltransferase	-
D1107_RS07715 (D1107_07800)	1684319..1684786	-	468	WP_237592683.1	GNAT family N-acetyltransferase	Toxin
D1107_RS07720 (D1107_07805)	1684790..1685050	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
D1107_RS07725 (D1107_07810)	1685171..1686631	-	1461	WP_237592684.1	metalloprotease TldD	-
D1107_RS07730 (D1107_07815)	1686761..1688020	+	1260	WP_005615928.1	tRNA lysidine(34) synthetase TilS	-
D1107_RS07735 (D1107_07820)	1688047..1688337	-	291	WP_005602215.1	DUF5389 family protein	-
D1107_RS07740 (D1107_07825)	1688339..1689232	-	894	WP_005598850.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17286.26 Da
• Isoelectric Point: 9.2515

>T110407 WP_237592683.1 NZ_CP031860:c1684786-1684319 [Actinobacillus pleuropneumoniae]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVCDENRQVMGFYCLSAGSVSRQFVAGSLRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGVKALLVHALNQQAKQFYLKYGFSCSPIDEMVLMLKL

Nucleotide

• Length: 468 bp

>T110407 NZ_CP031860:c1684786-1684319 [Actinobacillus pleuropneumoniae]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGTGTGATGAGAATAGACAGGTGATGG
GGTTTTATTGCTTATCTGCCGGTTCTGTGAGTCGTCAGTTTGTTGCAGGCTCATTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGCTCGGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTAGCGAATCAAATCGGGGTAAAAGCATTGCTTGTTCATGCGTTAAATCAGCAAGCAAAAC
AATTTTATTTAAAGTACGGTTTTTCATGTTCACCCATTGATGAGATGGTTTTAATGTTAAAACTTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT110407 WP_005619269.1 NZ_CP031860:c1685050-1684790 [Actinobacillus pleuropneumoniae]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT110407 NZ_CP031860:c1685050-1684790 [Actinobacillus pleuropneumoniae]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGCATTTTAGGTAA
AACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACCGTTTTCCAATTAA
ATGAGCAGGCTTTCGAGCAATTTAACCGATTGCTAGAACAGCCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Antitoxin

• AlphaFold DB: A3N2I7