Detailed information of TA system TA110404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2016202..2016917
• Replicon: chromosome
• Accession: NZ_CP031859
• Organism: Actinobacillus pleuropneumoniae strain HS143

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1108_RS09420
• Protein ID: WP_005599445.1
• Coordinates: 2016202..2016567 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: D1108_RS09425
• Protein ID: WP_005599447.1
• Coordinates: 2016630..2016917 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1108_RS09405 (D1108_09465)	2011787..2013493	-	1707	WP_237593408.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
D1108_RS09410 (D1108_09470)	2013589..2013870	-	282	WP_005609261.1	DNA-directed RNA polymerase subunit omega	-
D1108_RS09415 (D1108_09475)	2013997..2016078	-	2082	WP_237593409.1	ATP-dependent DNA helicase RecG	-
D1108_RS09420 (D1108_09480)	2016202..2016567	-	366	WP_005599445.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1108_RS09425 (D1108_09485)	2016630..2016917	-	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D1108_RS09430 (D1108_09490)	2017123..2018838	+	1716	WP_039709409.1	proline--tRNA ligase	-
D1108_RS09435 (D1108_09495)	2018904..2019092	-	189	WP_005602684.1	hypothetical protein	-
D1108_RS09440 (D1108_09500)	2019095..2019676	-	582	WP_005606496.1	sigma-70 family RNA polymerase sigma factor	-
D1108_RS09445 (D1108_09505)	2019765..2020721	+	957	WP_012263408.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
D1108_RS09450 (D1108_09510)	2020721..2021314	+	594	WP_005599453.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13956.02 Da
• Isoelectric Point: 9.0549

>T110404 WP_005599445.1 NZ_CP031859:c2016567-2016202 [Actinobacillus pleuropneumoniae]
IDTNVRQWLATIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQAKSFSINNEIALLASEYHIPNK
MDLNDAYIAATAKYHNLVLVTRNLKDFNRCDIRLFNPFEPN

Nucleotide

• Length: 366 bp

>T110404 NZ_CP031859:c2016567-2016202 [Actinobacillus pleuropneumoniae]
ATAGATACCAATGTACGACAATGGTTAGCAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAAT
AAAAACGGGGATTTTACTTAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTAT
TAAGCGTTTATCAAGCTAAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAA
ATGGATTTAAACGATGCTTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTT
CAACCGTTGCGATATACGTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT110404 WP_005599447.1 NZ_CP031859:c2016917-2016630 [Actinobacillus pleuropneumoniae]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT110404 NZ_CP031859:c2016917-2016630 [Actinobacillus pleuropneumoniae]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5