Detailed information of TA system TA110402

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1718123..1718854
• Replicon: chromosome
• Accession: NZ_CP031859
• Organism: Actinobacillus pleuropneumoniae strain HS143

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A448U107
• Locus tag: D1108_RS07850
• Protein ID: WP_005602211.1
• Coordinates: 1718123..1718590 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: D1108_RS07855
• Protein ID: WP_005619269.1
• Coordinates: 1718594..1718854 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1108_RS07825 (D1108_07865)	1713482..1713853	-	372	WP_005598830.1	CrcB family protein	-
D1108_RS07830 (D1108_07870)	1713856..1714920	-	1065	WP_039709309.1	MFS transporter	-
D1108_RS07835 (D1108_07875)	1715015..1716124	+	1110	WP_237593340.1	anhydro-N-acetylmuramic acid kinase	-
D1108_RS07840 (D1108_07880)	1716178..1717092	+	915	WP_009875244.1	N-acetylmuramic acid 6-phosphate etherase	-
D1108_RS07845 (D1108_07885)	1717159..1718040	-	882	WP_005598838.1	50S ribosomal protein L11 methyltransferase	-
D1108_RS07850 (D1108_07890)	1718123..1718590	-	468	WP_005602211.1	GNAT family N-acetyltransferase	Toxin
D1108_RS07855 (D1108_07895)	1718594..1718854	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
D1108_RS07860 (D1108_07900)	1718975..1720435	-	1461	WP_237593341.1	metalloprotease TldD	-
D1108_RS07865 (D1108_07905)	1720565..1721824	+	1260	WP_237593342.1	tRNA lysidine(34) synthetase TilS	-
D1108_RS07870 (D1108_07910)	1721851..1722063	-	213	WP_237593654.1	DUF5389 family protein	-
D1108_RS07875 (D1108_07915)	1722143..1723036	-	894	WP_237593343.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17192.19 Da
• Isoelectric Point: 8.9312

>T110402 WP_005602211.1 NZ_CP031859:c1718590-1718123 [Actinobacillus pleuropneumoniae]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVCDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGVKALLVHALNPQAKQFYLKYGFSCSPIDEMVLMLKL

Nucleotide

• Length: 468 bp

>T110402 NZ_CP031859:c1718590-1718123 [Actinobacillus pleuropneumoniae]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGTGTGATGAAAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGCCATCAGTTTGTTGCAGGTGCCTTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGTTCGGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTGGCGAACCAAATCGGGGTAAAAGCATTGCTTGTTCATGCGTTAAATCCGCAAGCAAAAC
AATTTTATTTAAAGTACGGTTTTTCATGTTCACCCATTGATGAGATGGTTTTAATGTTAAAACTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT110402 WP_005619269.1 NZ_CP031859:c1718854-1718594 [Actinobacillus pleuropneumoniae]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT110402 NZ_CP031859:c1718854-1718594 [Actinobacillus pleuropneumoniae]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGCATTTTAGGTAA
AACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACCGTTTTCCAATTAA
ATGAGCAGGCTTTCGAGCAATTTAACCGATTGCTAGAACAGCCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Toxin

• AlphaFold DB: A0A448U107

Antitoxin

• AlphaFold DB: A3N2I7