Detailed information of TA system TA110401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 1512190..1512835
• Replicon: chromosome
• Accession: NZ_CP031859
• Organism: Actinobacillus pleuropneumoniae strain HS143

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: B0BQU7
• Locus tag: D1108_RS06840
• Protein ID: WP_005619674.1
• Coordinates: 1512464..1512835 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: D1108_RS06835
• Protein ID: WP_005598477.1
• Coordinates: 1512190..1512483 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1108_RS06805 (D1108_06850)	1507522..1508205	-	684	WP_005605188.1	arginine ABC transporter permease ArtM	-
D1108_RS06810 (D1108_06855)	1508205..1508876	-	672	WP_237593313.1	arginine ABC transporter permease ArtQ	-
D1108_RS06815 (D1108_06860)	1508882..1509601	-	720	WP_005619670.1	transporter substrate-binding domain-containing protein	-
D1108_RS06820 (D1108_06865)	1509621..1510355	-	735	WP_005598473.1	arginine ABC transporter ATP-binding protein ArtP	-
D1108_RS06825 (D1108_06870)	1510580..1511077	-	498	WP_005601953.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
D1108_RS06830 (D1108_06875)	1511150..1512112	+	963	WP_005619672.1	calcium/sodium antiporter	-
D1108_RS06835 (D1108_06880)	1512190..1512483	-	294	WP_005598477.1	helix-turn-helix domain-containing protein	Antitoxin
D1108_RS06840 (D1108_06885)	1512464..1512835	-	372	WP_005619674.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D1108_RS06845 (D1108_06890)	1513029..1514780	+	1752	WP_237593314.1	protein-disulfide reductase DsbD	-
D1108_RS06850 (D1108_06895)	1514838..1515407	+	570	WP_005605204.1	elongation factor P hydroxylase	-
D1108_RS06855 (D1108_06900)	1515451..1516305	-	855	WP_005598486.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (32-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-85)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 15066.57 Da
• Isoelectric Point: 10.4054

>T110401 WP_005619674.1 NZ_CP031859:c1512835-1512464 [Actinobacillus pleuropneumoniae]
MYEIVFYRDKRGREPVKEFLLRLLKERQEGSRERLHKISHHLSILHLHGTRAGENYIKHLEDRIWQLRPVGDCLLFASII
RGKFVLLHYFAKQNYRIPKREIERAKVRLADLQERIKDEPYWF

Nucleotide

• Length: 372 bp

>T110401 NZ_CP031859:c1512835-1512464 [Actinobacillus pleuropneumoniae]
ATGTATGAAATAGTCTTTTATCGAGATAAGCGGGGGAGAGAACCCGTAAAAGAATTTCTGTTAAGGCTACTAAAAGAACG
GCAGGAAGGCAGTCGAGAACGATTACACAAAATCAGCCATCATTTGAGTATTTTACATTTGCACGGTACTCGAGCCGGTG
AGAATTATATTAAGCATTTGGAAGATCGTATTTGGCAGTTACGTCCGGTGGGCGATTGCCTGTTATTTGCAAGTATTATT
CGGGGAAAATTTGTGTTGTTGCATTATTTTGCCAAACAGAATTATCGAATTCCGAAACGAGAAATAGAACGTGCAAAAGT
TCGGTTGGCGGATTTACAGGAGAGAATAAAAGATGAGCCCTATTGGTTCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10935.81 Da
• Isoelectric Point: 10.3120

>AT110401 WP_005598477.1 NZ_CP031859:c1512483-1512190 [Actinobacillus pleuropneumoniae]
MSPIGSSWTSFEQEIFDESEIAETQFRIRLILEIIETRQQLGISQRKLEKLSGVKQSMIARVEKGSVNPSLATVLKLLVP
LGKTLQIVPLDKNKTVR

Nucleotide

• Length: 294 bp

>AT110401 NZ_CP031859:c1512483-1512190 [Actinobacillus pleuropneumoniae]
ATGAGCCCTATTGGTTCTAGTTGGACATCGTTTGAACAAGAAATTTTTGACGAGTCGGAAATAGCGGAAACTCAGTTTCG
CATACGACTTATTTTAGAAATCATTGAAACCCGTCAGCAGCTCGGTATTTCTCAACGAAAATTGGAGAAATTAAGCGGCG
TAAAACAATCTATGATTGCTCGGGTAGAAAAAGGCTCGGTTAATCCGTCGTTGGCGACGGTATTAAAGTTGCTTGTGCCG
TTGGGGAAAACTTTACAAATCGTACCGTTAGATAAAAACAAAACGGTACGTTAA

Structures

Toxin

• AlphaFold DB: A3N206