Detailed information of TA system TA110399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 726302..726952
• Replicon: chromosome
• Accession: NZ_CP031859
• Organism: Actinobacillus pleuropneumoniae strain HS143

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B0BNU3
• Locus tag: D1108_RS03245
• Protein ID: WP_005614926.1
• Coordinates: 726302..726691 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BNU4
• Locus tag: D1108_RS03250
• Protein ID: WP_005604117.1
• Coordinates: 726692..726952 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1108_RS03235 (D1108_03235)	721542..724817	-	3276	WP_237593579.1	helicase-related protein	-
D1108_RS03240 (D1108_03240)	724943..726166	-	1224	WP_012262907.1	ATP-binding protein	-
D1108_RS03245 (D1108_03245)	726302..726691	-	390	WP_005614926.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1108_RS03250 (D1108_03250)	726692..726952	-	261	WP_005604117.1	hypothetical protein	Antitoxin
D1108_RS03255 (D1108_03255)	727298..728134	+	837	WP_012262908.1	DUF6216 family protein	-
D1108_RS03260 (D1108_03260)	728166..730187	-	2022	WP_012262909.1	excinuclease ABC subunit UvrB	-
D1108_RS03265 (D1108_03270)	730364..731176	+	813	WP_005607533.1	iron uptake system protein EfeO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14837.17 Da
• Isoelectric Point: 5.1295

>T110399 WP_005614926.1 NZ_CP031859:c726691-726302 [Actinobacillus pleuropneumoniae]
MYMLDTNTVSYFFRQDPTVVKKLQQLNPELICISSVTAAELFYGVKKRNNQKLTAFLNTFLSAITVMDWDYQVAEVYGQL
RAEMEREGKIMGVQDQMIGAHALATECVLVSSDKAFQFIPNLVLENWWK

Nucleotide

• Length: 390 bp

>T110399 NZ_CP031859:c726691-726302 [Actinobacillus pleuropneumoniae]
ATGTATATGCTAGATACTAATACGGTGAGTTATTTCTTTAGGCAAGATCCTACTGTGGTTAAGAAACTACAACAACTTAA
TCCGGAATTGATTTGTATTTCATCGGTCACGGCTGCAGAGTTGTTTTATGGCGTAAAAAAACGTAATAATCAGAAACTTA
CTGCATTTTTAAATACTTTTTTATCTGCGATTACGGTGATGGATTGGGATTATCAAGTGGCGGAAGTTTATGGTCAGCTG
CGAGCAGAGATGGAACGAGAAGGCAAAATCATGGGCGTTCAAGATCAAATGATTGGAGCTCATGCCCTAGCAACAGAATG
TGTTCTTGTTTCCAGCGATAAAGCCTTTCAATTTATCCCTAATCTCGTATTAGAAAATTGGTGGAAGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10289.63 Da
• Isoelectric Point: 5.1867

>AT110399 WP_005604117.1 NZ_CP031859:c726952-726692 [Actinobacillus pleuropneumoniae]
MEKVARLFRNGRNQAVRLPVEFEFNAKQVYVFKEANGDITLSTRSRNQQNWQKVFELLPMLECDEQFLSEQERKQEMATR
DPFEGF

Nucleotide

• Length: 261 bp

>AT110399 NZ_CP031859:c726952-726692 [Actinobacillus pleuropneumoniae]
ATGGAAAAGGTCGCACGTTTATTTAGAAACGGACGTAATCAAGCCGTACGATTACCGGTTGAATTTGAGTTTAATGCGAA
GCAAGTTTATGTGTTTAAAGAAGCAAACGGCGATATTACGCTTTCGACACGTTCTCGCAATCAGCAAAATTGGCAGAAAG
TCTTTGAGTTATTACCAATGCTTGAATGTGATGAACAGTTTTTATCGGAACAAGAACGCAAGCAAGAAATGGCAACTCGA
GATCCGTTCGAGGGCTTTTAA

Structures

Toxin

• AlphaFold DB: A3N029

Antitoxin

• AlphaFold DB: A3N030