Detailed information of TA system TA110398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 1498575..1499290
• Replicon: chromosome
• Accession: NZ_CP031858
• Organism: Actinobacillus pleuropneumoniae strain A-85/14

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1109_RS07290
• Protein ID: WP_005599445.1
• Coordinates: 1498575..1498940 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: D1109_RS07295
• Protein ID: WP_005599447.1
• Coordinates: 1499003..1499290 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1109_RS07275 (D1109_07295)	1494160..1495866	-	1707	WP_237610950.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
D1109_RS07280 (D1109_07300)	1495962..1496243	-	282	WP_012263402.1	DNA-directed RNA polymerase subunit omega	-
D1109_RS07285 (D1109_07305)	1496370..1498451	-	2082	WP_005613379.1	ATP-dependent DNA helicase RecG	-
D1109_RS07290 (D1109_07310)	1498575..1498940	-	366	WP_005599445.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1109_RS07295 (D1109_07315)	1499003..1499290	-	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D1109_RS07300 (D1109_07320)	1499496..1501211	+	1716	WP_237610951.1	proline--tRNA ligase	-
D1109_RS07305 (D1109_07325)	1501277..1501465	-	189	WP_005602684.1	hypothetical protein	-
D1109_RS07310 (D1109_07330)	1501468..1502049	-	582	WP_005606496.1	sigma-70 family RNA polymerase sigma factor	-
D1109_RS07315 (D1109_07335)	1502137..1503093	+	957	WP_237610952.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
D1109_RS07320 (D1109_07340)	1503093..1503686	+	594	WP_005599453.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13974.06 Da
• Isoelectric Point: 9.0549

>T110398 WP_005599445.1 NZ_CP031858:c1498940-1498575 [Actinobacillus pleuropneumoniae]
MDTNVRQWLATIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQAKSFSINNEIALLASEYHIPNK
MDLNDAYIAATAKYHNLVLVTRNLKDFNRCDIRLFNPFEPN

Nucleotide

• Length: 366 bp

>T110398 NZ_CP031858:c1498940-1498575 [Actinobacillus pleuropneumoniae]
ATGGATACCAATGTACGACAATGGTTAGCAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAAT
AAAAACGGGGATTTTACTTAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTAT
TAAGCGTTTATCAAGCTAAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAA
ATGGATTTAAACGATGCTTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTT
CAACCGTTGCGATATACGTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT110398 WP_005599447.1 NZ_CP031858:c1499290-1499003 [Actinobacillus pleuropneumoniae]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT110398 NZ_CP031858:c1499290-1499003 [Actinobacillus pleuropneumoniae]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5