Detailed information of TA system TA110397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1413565..1414193
• Replicon: chromosome
• Accession: NZ_CP031858
• Organism: Actinobacillus pleuropneumoniae strain A-85/14

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1109_RS06770
• Protein ID: WP_237610933.1
• Coordinates: 1413795..1414193 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B3GYV7
• Locus tag: D1109_RS06765
• Protein ID: WP_005613300.1
• Coordinates: 1413565..1413795 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1109_RS06755 (D1109_06770)	1410873..1412423	+	1551	WP_005616128.1	class I SAM-dependent methyltransferase	-
D1109_RS06760 (D1109_06775)	1412420..1413202	+	783	WP_237610932.1	HincII family type II restriction endonuclease	-
D1109_RS06765 (D1109_06780)	1413565..1413795	+	231	WP_005613300.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D1109_RS06770 (D1109_06785)	1413795..1414193	+	399	WP_237610933.1	tRNA(fMet)-specific endonuclease VapC	Toxin
D1109_RS06775 (D1109_06790)	1414282..1415615	+	1334	WP_237610934.1	IS3 family transposase	-
D1109_RS06780 (D1109_06795)	1415750..1416166	-	417	WP_005599244.1	DUF417 family protein	-
D1109_RS06785 (D1109_06800)	1416403..1417593	-	1191	WP_005599245.1	pentapeptide repeat-containing protein	-
D1109_RS06790 (D1109_06805)	1417646..1418968	-	1323	WP_237610935.1	HslU--HslV peptidase ATPase subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1397167..1417593	20426

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-53)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15119.42 Da
• Isoelectric Point: 7.4195

>T110397 WP_237610933.1 NZ_CP031858:1413795-1414193 [Actinobacillus pleuropneumoniae]
MLTYMLDTNIAIYVIKRRPIEVLDKFNLNSTRLCVSSITAAELYYGAEKSQFPERNMVVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKQGKIIGENDIHIAAHARSEGLVLVTNNLREFERVEGLRLDNWV

Nucleotide

• Length: 399 bp

>T110397 NZ_CP031858:1413795-1414193 [Actinobacillus pleuropneumoniae]
ATGCTGACTTATATGCTTGATACTAATATTGCGATTTATGTGATTAAACGCAGACCTATTGAAGTGCTGGATAAGTTTAA
TTTAAATTCTACTCGTCTTTGTGTAAGTAGTATCACTGCAGCGGAGTTGTATTATGGTGCAGAGAAAAGCCAGTTTCCGG
AACGAAATATGGTAGTCATTGAGGATTTTCTTTCTCGTTTAACGATTCTTGATTATACGCATAAGGCAGCTACTCACTTT
GGCAATATCAAAGCACATTTATCTAAGCAAGGTAAGATCATTGGCGAGAACGATATTCATATTGCCGCACATGCAAGAAG
TGAAGGACTCGTATTAGTGACCAATAACTTGCGTGAATTTGAACGTGTGGAAGGATTGCGATTAGATAATTGGGTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8625.71 Da
• Isoelectric Point: 4.5358

>AT110397 WP_005613300.1 NZ_CP031858:1413565-1413795 [Actinobacillus pleuropneumoniae]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLSSATVSDDFMTEREISTQAEREEL

Nucleotide

• Length: 231 bp

>AT110397 NZ_CP031858:1413565-1413795 [Actinobacillus pleuropneumoniae]
ATGGAAGCATCAGTATTTATGACAAATCGCAGTCAAGCGGTACGATTGCCGGCAGAAGTACGTTTTGCAAGTGATGTAAA
AAAACTTTCTGTAAGAGTAGTTGGTAATGAGCGTATTTTGGCACCGATTAACCAAAGTTGGGATAGCTTCTTTCTGAGTT
CGGCAACGGTAAGCGATGATTTTATGACAGAGCGTGAAATCAGCACTCAAGCTGAACGTGAGGAACTGTAA

Structures

Antitoxin

• AlphaFold DB: A3N328