Detailed information of TA system TA110395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 995575..996220
• Replicon: chromosome
• Accession: NZ_CP031858
• Organism: Actinobacillus pleuropneumoniae strain A-85/14

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: B0BQU7
• Locus tag: D1109_RS04695
• Protein ID: WP_005619674.1
• Coordinates: 995849..996220 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: D1109_RS04690
• Protein ID: WP_005598477.1
• Coordinates: 995575..995868 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1109_RS04660 (D1109_04685)	990907..991590	-	684	WP_005608586.1	arginine ABC transporter permease ArtM	-
D1109_RS04665 (D1109_04690)	991590..992261	-	672	WP_005612835.1	arginine ABC transporter permease ArtQ	-
D1109_RS04670 (D1109_04695)	992267..992986	-	720	WP_011848574.1	transporter substrate-binding domain-containing protein	-
D1109_RS04675 (D1109_04700)	993006..993740	-	735	WP_005598473.1	arginine ABC transporter ATP-binding protein ArtP	-
D1109_RS04680 (D1109_04705)	993965..994462	-	498	WP_005601953.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
D1109_RS04685 (D1109_04710)	994535..995497	+	963	WP_005619672.1	calcium/sodium antiporter	-
D1109_RS04690 (D1109_04715)	995575..995868	-	294	WP_005598477.1	helix-turn-helix domain-containing protein	Antitoxin
D1109_RS04695 (D1109_04720)	995849..996220	-	372	WP_005619674.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D1109_RS04700 (D1109_04725)	996413..998164	+	1752	WP_237611441.1	protein-disulfide reductase DsbD	-
D1109_RS04705 (D1109_04730)	998222..998791	+	570	WP_005601959.1	elongation factor P hydroxylase	-
D1109_RS04710 (D1109_04735)	998835..999689	-	855	WP_009874917.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (32-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-85)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 15066.57 Da
• Isoelectric Point: 10.4054

>T110395 WP_005619674.1 NZ_CP031858:c996220-995849 [Actinobacillus pleuropneumoniae]
MYEIVFYRDKRGREPVKEFLLRLLKERQEGSRERLHKISHHLSILHLHGTRAGENYIKHLEDRIWQLRPVGDCLLFASII
RGKFVLLHYFAKQNYRIPKREIERAKVRLADLQERIKDEPYWF

Nucleotide

• Length: 372 bp

>T110395 NZ_CP031858:c996220-995849 [Actinobacillus pleuropneumoniae]
ATGTATGAAATAGTCTTTTATCGAGATAAGCGGGGGAGAGAACCCGTAAAAGAATTTCTGTTAAGGCTGCTAAAAGAACG
ACAGGAAGGCAGTCGAGAACGATTACACAAAATCAGCCATCATTTGAGTATTTTACATTTGCACGGTACTCGAGCCGGTG
AGAATTATATTAAGCATTTGGAAGATCGTATTTGGCAGTTACGTCCGGTGGGCGATTGCCTGTTATTTGCAAGTATTATT
CGGGGAAAATTTGTGTTGTTGCATTATTTTGCCAAACAGAATTATCGAATTCCGAAACGAGAAATAGAACGTGCAAAAGT
TCGGTTGGCGGATTTACAGGAGAGAATAAAAGATGAGCCCTATTGGTTCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10935.81 Da
• Isoelectric Point: 10.3120

>AT110395 WP_005598477.1 NZ_CP031858:c995868-995575 [Actinobacillus pleuropneumoniae]
MSPIGSSWTSFEQEIFDESEIAETQFRIRLILEIIETRQQLGISQRKLEKLSGVKQSMIARVEKGSVNPSLATVLKLLVP
LGKTLQIVPLDKNKTVR

Nucleotide

• Length: 294 bp

>AT110395 NZ_CP031858:c995868-995575 [Actinobacillus pleuropneumoniae]
ATGAGCCCTATTGGTTCTAGTTGGACATCGTTTGAACAAGAAATTTTTGACGAGTCGGAAATAGCGGAAACTCAGTTTCG
CATACGACTTATTTTAGAAATCATTGAAACCCGTCAGCAGCTCGGTATTTCTCAACGAAAATTGGAGAAATTAAGCGGCG
TAAAACAATCTATGATTGCTCGGGTAGAAAAAGGCTCGGTTAATCCGTCGTTGGCGACGGTATTAAAGTTGCTTGTGCCG
TTGGGGAAAACTTTACAAATCGTACCGTTAGATAAAAACAAAACGGTACGTTAA

Structures

Toxin

• AlphaFold DB: A3N206