Detailed information of TA system TA110394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 908188..908696
• Replicon: chromosome
• Accession: NZ_CP031858
• Organism: Actinobacillus pleuropneumoniae strain A-85/14

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: D1109_RS04235
• Protein ID: WP_237611416.1
• Coordinates: 908436..908696 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B0BQK8
• Locus tag: D1109_RS04230
• Protein ID: WP_005598318.1
• Coordinates: 908188..908439 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1109_RS04190 (D1109_04210)	903724..904272	-	549	WP_011848557.1	type IV pilus biogenesis/stability protein PilW	-
D1109_RS04195 (D1109_04215)	904326..905507	-	1182	WP_009875290.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
D1109_RS04220 (D1109_04240)	906411..907850	+	1440	WP_005605060.1	glutamate--tRNA ligase	-
D1109_RS04230 (D1109_04250)	908188..908439	+	252	WP_005598318.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
D1109_RS04235 (D1109_04255)	908436..908696	+	261	WP_237611416.1	Txe/YoeB family addiction module toxin	Toxin
D1109_RS04240 (D1109_04260)	908777..909986	+	1210	Protein_836	IS3 family transposase	-
D1109_RS04245 (D1109_04265)	910152..910319	-	168	WP_009875287.1	Trm112 family protein	-
D1109_RS04250 (D1109_04270)	910321..911301	-	981	WP_237611417.1	tetraacyldisaccharide 4'-kinase	-
D1109_RS04255 (D1109_04275)	911395..912654	-	1260	WP_005598324.1	ATP-dependent protease ATP-binding subunit ClpX	-
D1109_RS04260 (D1109_04280)	912654..913244	-	591	WP_005601821.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10407.94 Da
• Isoelectric Point: 8.0109

>T110394 WP_237611416.1 NZ_CP031858:908436-908696 [Actinobacillus pleuropneumoniae]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKPEPLKFNLSGFWSRRINEEHRLIYSVEDEAILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T110394 NZ_CP031858:908436-908696 [Actinobacillus pleuropneumoniae]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACCGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9362.66 Da
• Isoelectric Point: 4.5659

>AT110394 WP_005598318.1 NZ_CP031858:908188-908439 [Actinobacillus pleuropneumoniae]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYCSLEETAYLMRSPTNAKRLLDSVAELRQGQGIERE
LIE

Nucleotide

• Length: 252 bp

>AT110394 NZ_CP031858:908188-908439 [Actinobacillus pleuropneumoniae]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACGATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TGATGCGTTCACCCACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Antitoxin

• AlphaFold DB: B0BQK8