Detailed information of TA system TA110391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1973000..1973628
• Replicon: chromosome
• Accession: NZ_CP031856
• Organism: Actinobacillus pleuropneumoniae strain 16287-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1110_RS09240
• Protein ID: WP_237585957.1
• Coordinates: 1973230..1973628 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BSF9
• Locus tag: D1110_RS09235
• Protein ID: WP_005616131.1
• Coordinates: 1973000..1973230 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1110_RS09225 (D1110_09310)	1970848..1972479	+	1632	WP_237585956.1	hypothetical protein	-
D1110_RS09230	1972752..1972892	-	141	WP_162835170.1	hypothetical protein	-
D1110_RS09235 (D1110_09315)	1973000..1973230	+	231	WP_005616131.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D1110_RS09240 (D1110_09320)	1973230..1973628	+	399	WP_237585957.1	tRNA(fMet)-specific endonuclease VapC	Toxin
D1110_RS09245 (D1110_09325)	1973764..1974180	-	417	WP_005602541.1	DUF417 family protein	-
D1110_RS09250 (D1110_09330)	1974426..1975076	+	651	WP_237585958.1	DUF2202 domain-containing protein	-
D1110_RS09255 (D1110_09335)	1975151..1975810	-	660	WP_237585959.1	hypothetical protein	-
D1110_RS09260 (D1110_09340)	1976124..1977446	-	1323	WP_018652450.1	HslU--HslV peptidase ATPase subunit	-
D1110_RS09265 (D1110_09345)	1977501..1978022	-	522	WP_005618211.1	ATP-dependent protease subunit HslV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1958583..1988410	29827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15063.42 Da
• Isoelectric Point: 8.0810

>T110391 WP_237585957.1 NZ_CP031856:1973230-1973628 [Actinobacillus pleuropneumoniae]
MLTYMLDTNIAIYVIKRRPIEVLDKFNLNSTRLCVSSITAAELYYGAEKSQFPERNMAVIEDFLSRLTILDYMHKAATHF
GNIKAHLSKQGKIIGENDIHIAAHARSEGLVLVTNNLRAFERVEGLRLDNWV

Nucleotide

• Length: 399 bp

>T110391 NZ_CP031856:1973230-1973628 [Actinobacillus pleuropneumoniae]
ATGCTAACTTATATGCTTGATACTAACATTGCGATTTATGTGATTAAACGCAGACCTATTGAAGTGCTGGATAAGTTTAA
TTTAAATTCTACTCGTCTTTGTGTAAGTAGTATCACTGCAGCGGAGCTGTATTATGGGGCAGAGAAAAGCCAGTTTCCGG
AACGAAATATGGCAGTCATTGAGGATTTTCTTTCTCGTTTAACGATTCTTGATTATATGCATAAGGCGGCTACTCACTTT
GGCAATATCAAAGCACATTTATCTAAGCAAGGTAAGATCATTGGCGAGAACGATATTCATATTGCCGCACATGCAAGAAG
TGAAGGACTCGTATTAGTGACCAATAACTTGCGTGCATTTGAACGTGTGGAAGGATTGCGATTAGATAATTGGGTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8595.68 Da
• Isoelectric Point: 4.5358

>AT110391 WP_005616131.1 NZ_CP031856:1973000-1973230 [Actinobacillus pleuropneumoniae]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLGSATVSDDFMTEREISTQAEREEL

Nucleotide

• Length: 231 bp

>AT110391 NZ_CP031856:1973000-1973230 [Actinobacillus pleuropneumoniae]
ATGGAAGCATCAGTATTTATGACAAATCGCAGTCAAGCGGTACGATTGCCGGCAGAAGTACGTTTTGCAAGTGATGTAAA
AAAACTTTCTGTAAGAGTGGTAGGTAATGAACGTATTTTGGCACCGATTAACCAAAGTTGGGATAGCTTCTTTCTGGGGT
CGGCAACGGTAAGCGATGATTTCATGACAGAGCGTGAAATCAGCACTCAAGCCGAACGTGAGGAACTATAA

Structures

Antitoxin

• AlphaFold DB: B0BSF9