Detailed information of TA system TA110388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 1465272..1465780
• Replicon: chromosome
• Accession: NZ_CP031856
• Organism: Actinobacillus pleuropneumoniae strain 16287-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B0BQK9
• Locus tag: D1110_RS06705
• Protein ID: WP_005605064.1
• Coordinates: 1465520..1465780 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B0BQK8
• Locus tag: D1110_RS06700
• Protein ID: WP_005598318.1
• Coordinates: 1465272..1465523 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1110_RS06660 (D1110_06710)	1460808..1461356	-	549	WP_237585836.1	type IV pilus biogenesis/stability protein PilW	-
D1110_RS06665 (D1110_06715)	1461410..1462591	-	1182	WP_005598300.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
D1110_RS06690 (D1110_06740)	1463495..1464934	+	1440	WP_005605060.1	glutamate--tRNA ligase	-
D1110_RS06700 (D1110_06750)	1465272..1465523	+	252	WP_005598318.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
D1110_RS06705 (D1110_06755)	1465520..1465780	+	261	WP_005605064.1	Txe/YoeB family addiction module toxin	Toxin
D1110_RS06710 (D1110_06760)	1465939..1466106	-	168	WP_005598320.1	Trm112 family protein	-
D1110_RS06715 (D1110_06765)	1466108..1467088	-	981	WP_005598322.1	tetraacyldisaccharide 4'-kinase	-
D1110_RS06720 (D1110_06770)	1467182..1468441	-	1260	WP_005598324.1	ATP-dependent protease ATP-binding subunit ClpX	-
D1110_RS06725 (D1110_06775)	1468441..1469031	-	591	WP_005598326.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
D1110_RS06730 (D1110_06780)	1469153..1469545	+	393	WP_005620423.1	SufE family protein	-
D1110_RS06745 (D1110_06795)	1469904..1470677	-	774	WP_005618837.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10423.99 Da
• Isoelectric Point: 8.0109

>T110388 WP_005605064.1 NZ_CP031856:1465520-1465780 [Actinobacillus pleuropneumoniae]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKLEPLKFNLSGFWSRRINEEHRLIYSVEDEAILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T110388 NZ_CP031856:1465520-1465780 [Actinobacillus pleuropneumoniae]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9362.66 Da
• Isoelectric Point: 4.5659

>AT110388 WP_005598318.1 NZ_CP031856:1465272-1465523 [Actinobacillus pleuropneumoniae]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYCSLEETAYLMRSPTNAKRLLDSVAELRQGQGIERE
LIE

Nucleotide

• Length: 252 bp

>AT110388 NZ_CP031856:1465272-1465523 [Actinobacillus pleuropneumoniae]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACGATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TGATGCGTTCACCCACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Toxin

• AlphaFold DB: B0BQK9

Antitoxin

• AlphaFold DB: B0BQK8