Detailed information of TA system TA110385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1907184..1907812
• Replicon: chromosome
• Accession: NZ_CP031855
• Organism: Actinobacillus pleuropneumoniae strain 7311555

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1111_RS08830
• Protein ID: WP_215577974.1
• Coordinates: 1907414..1907812 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: D1111_RS08825
• Protein ID: WP_039709385.1
• Coordinates: 1907184..1907414 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1111_RS08815 (D1111_08885)	1905032..1906663	+	1632	WP_237610332.1	hypothetical protein	-
D1111_RS08820	1906936..1907109	-	174	Protein_1696	addiction module antidote protein, HigA family	-
D1111_RS08825 (D1111_08890)	1907184..1907414	+	231	WP_039709385.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D1111_RS08830 (D1111_08895)	1907414..1907812	+	399	WP_215577974.1	tRNA(fMet)-specific endonuclease VapC	Toxin
D1111_RS08835 (D1111_08900)	1907947..1908363	-	417	WP_237610333.1	DUF417 family protein	-
D1111_RS08840 (D1111_08905)	1908609..1909259	+	651	WP_237610334.1	DUF2202 domain-containing protein	-
D1111_RS08845 (D1111_08910)	1909332..1909991	-	660	WP_005605767.1	hypothetical protein	-
D1111_RS08850 (D1111_08915)	1910305..1911627	-	1323	WP_018652450.1	HslU--HslV peptidase ATPase subunit	-
D1111_RS08855 (D1111_08920)	1911682..1912203	-	522	WP_005618211.1	ATP-dependent protease subunit HslV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1892503..1909991	17488

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15091.37 Da
• Isoelectric Point: 7.4195

>T110385 WP_215577974.1 NZ_CP031855:1907414-1907812 [Actinobacillus pleuropneumoniae]
MLTYMLDTNIAIYVIKRRPIEVLDKFNLNSTRLCVSSITAAELYYGAEKSQFPERNMAVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKQGKIIGENDIHIAAHARSEGLVLVTNNLREFERVEGLRLDNWV

Nucleotide

• Length: 399 bp

>T110385 NZ_CP031855:1907414-1907812 [Actinobacillus pleuropneumoniae]
ATGCTAACTTATATGCTTGATACTAACATTGCGATTTATGTGATTAAACGCAGACCTATTGAAGTGCTGGATAAGTTTAA
TTTAAATTCTACTCGTCTTTGTGTAAGTAGTATCACTGCAGCGGAGTTGTATTATGGTGCAGAGAAAAGCCAGTTTCCGG
AACGAAATATGGCAGTAATTGAGGATTTTCTTTCTCGTTTAACGATTCTTGATTATACGCATAAGGCGGCTACTCACTTT
GGCAATATCAAAGCACATTTATCTAAGCAAGGTAAGATCATTGGCGAGAACGATATTCATATTGCCGCACATGCAAGAAG
TGAAGGACTCGTATTAGTGACCAATAACTTGCGTGAATTTGAACGTGTGGAAGGATTGCGATTAGATAATTGGGTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8581.65 Da
• Isoelectric Point: 4.5203

>AT110385 WP_039709385.1 NZ_CP031855:1907184-1907414 [Actinobacillus pleuropneumoniae]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLGSATVSDDFMTEREISTQAEREDL

Nucleotide

• Length: 231 bp

>AT110385 NZ_CP031855:1907184-1907414 [Actinobacillus pleuropneumoniae]
ATGGAAGCATCGGTATTTATGACAAACCGTAGTCAAGCGGTACGATTGCCGGCAGAAGTACGCTTTGCAAGTGATGTAAA
AAAACTTTCTGTAAGAGTAGTCGGTAATGAGCGTATTTTGGCTCCGATTAACCAAAGTTGGGATAGCTTCTTTCTGGGGT
CGGCAACGGTAAGCGATGATTTCATGACAGAGCGTGAAATCAGCACTCAAGCCGAACGTGAGGATCTATAA