Detailed information of TA system TA110383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1695045..1695776
• Replicon: chromosome
• Accession: NZ_CP031855
• Organism: Actinobacillus pleuropneumoniae strain 7311555

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: D1111_RS07790
• Protein ID: WP_005598840.1
• Coordinates: 1695045..1695512 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: D1111_RS07795
• Protein ID: WP_005619269.1
• Coordinates: 1695516..1695776 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1111_RS07765 (D1111_07825)	1690405..1690776	-	372	WP_005598830.1	CrcB family protein	-
D1111_RS07770 (D1111_07830)	1690779..1691843	-	1065	WP_005602204.1	MFS transporter	-
D1111_RS07775 (D1111_07835)	1691938..1693047	+	1110	WP_237610273.1	anhydro-N-acetylmuramic acid kinase	-
D1111_RS07780 (D1111_07840)	1693100..1694014	+	915	WP_237610274.1	N-acetylmuramic acid 6-phosphate etherase	-
D1111_RS07785 (D1111_07845)	1694081..1694962	-	882	WP_237610275.1	50S ribosomal protein L11 methyltransferase	-
D1111_RS07790 (D1111_07850)	1695045..1695512	-	468	WP_005598840.1	GNAT family N-acetyltransferase	Toxin
D1111_RS07795 (D1111_07855)	1695516..1695776	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
D1111_RS07800 (D1111_07860)	1695897..1697357	-	1461	WP_005598844.1	metalloprotease TldD	-
D1111_RS07805 (D1111_07865)	1697487..1698746	+	1260	WP_005615928.1	tRNA lysidine(34) synthetase TilS	-
D1111_RS07810 (D1111_07870)	1698773..1699063	-	291	WP_005602215.1	DUF5389 family protein	-
D1111_RS07815 (D1111_07875)	1699065..1699958	-	894	WP_005598850.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17206.22 Da
• Isoelectric Point: 8.9312

>T110383 WP_005598840.1 NZ_CP031855:c1695512-1695045 [Actinobacillus pleuropneumoniae]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVCDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGIKALLVHALNPQAKQFYLKYGFSCSPIDEMVLMLKL

Nucleotide

• Length: 468 bp

>T110383 NZ_CP031855:c1695512-1695045 [Actinobacillus pleuropneumoniae]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGTGTGATGAAAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGCCATCAGTTTGTTGCAGGTGCCTTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGTTCGGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTGGCGAACCAAATCGGGATAAAAGCATTGCTTGTTCATGCGTTAAACCCGCAAGCAAAAC
AATTTTATTTAAAGTATGGTTTTTCATGTTCACCCATTGATGAGATGGTATTAATGTTAAAACTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT110383 WP_005619269.1 NZ_CP031855:c1695776-1695516 [Actinobacillus pleuropneumoniae]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT110383 NZ_CP031855:c1695776-1695516 [Actinobacillus pleuropneumoniae]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGCATTTTAGGTAA
AACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACCGTTTTCCAATTAA
ATGAGCAGGCTTTCGAGCAATTTAACCGATTGCTAGAACAGCCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Antitoxin

• AlphaFold DB: A3N2I7