Detailed information of TA system TA110381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 1403236..1403744
• Replicon: chromosome
• Accession: NZ_CP031855
• Organism: Actinobacillus pleuropneumoniae strain 7311555

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B0BQK9
• Locus tag: D1111_RS06315
• Protein ID: WP_005605064.1
• Coordinates: 1403484..1403744 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B0BQK8
• Locus tag: D1111_RS06310
• Protein ID: WP_005598318.1
• Coordinates: 1403236..1403487 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1111_RS06270 (D1111_06320)	1398772..1399320	-	549	WP_237593742.1	type IV pilus biogenesis/stability protein PilW	-
D1111_RS06275 (D1111_06325)	1399374..1400555	-	1182	WP_005608495.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
D1111_RS06300 (D1111_06350)	1401459..1402898	+	1440	WP_237610207.1	glutamate--tRNA ligase	-
D1111_RS06310 (D1111_06360)	1403236..1403487	+	252	WP_005598318.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
D1111_RS06315 (D1111_06365)	1403484..1403744	+	261	WP_005605064.1	Txe/YoeB family addiction module toxin	Toxin
D1111_RS06320 (D1111_06370)	1403903..1404070	-	168	WP_005598320.1	Trm112 family protein	-
D1111_RS06325 (D1111_06375)	1404072..1405052	-	981	WP_005608502.1	tetraacyldisaccharide 4'-kinase	-
D1111_RS06330 (D1111_06380)	1405146..1406405	-	1260	WP_005598324.1	ATP-dependent protease ATP-binding subunit ClpX	-
D1111_RS06335 (D1111_06385)	1406405..1406995	-	591	WP_005598326.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
D1111_RS06340 (D1111_06390)	1407117..1407509	+	393	WP_005620423.1	SufE family protein	-
D1111_RS06355 (D1111_06405)	1407868..1408641	-	774	WP_005618837.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10423.99 Da
• Isoelectric Point: 8.0109

>T110381 WP_005605064.1 NZ_CP031855:1403484-1403744 [Actinobacillus pleuropneumoniae]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKLEPLKFNLSGFWSRRINEEHRLIYSVEDEAILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T110381 NZ_CP031855:1403484-1403744 [Actinobacillus pleuropneumoniae]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9362.66 Da
• Isoelectric Point: 4.5659

>AT110381 WP_005598318.1 NZ_CP031855:1403236-1403487 [Actinobacillus pleuropneumoniae]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYCSLEETAYLMRSPTNAKRLLDSVAELRQGQGIERE
LIE

Nucleotide

• Length: 252 bp

>AT110381 NZ_CP031855:1403236-1403487 [Actinobacillus pleuropneumoniae]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACGATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TGATGCGTTCACCCACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Toxin

• AlphaFold DB: B0BQK9

Antitoxin

• AlphaFold DB: B0BQK8