Detailed information of TA system TA110380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2017036..2017751
• Replicon: chromosome
• Accession: NZ_CP031854
• Organism: Actinobacillus pleuropneumoniae strain ORG1224

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1112_RS09430
• Protein ID: WP_052290829.1
• Coordinates: 2017036..2017401 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: D1112_RS09435
• Protein ID: WP_005599447.1
• Coordinates: 2017464..2017751 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1112_RS09415 (D1112_09495)	2012621..2014327	-	1707	WP_005606484.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
D1112_RS09420 (D1112_09500)	2014423..2014704	-	282	WP_012263402.1	DNA-directed RNA polymerase subunit omega	-
D1112_RS09425 (D1112_09505)	2014831..2016912	-	2082	WP_012263403.1	ATP-dependent DNA helicase RecG	-
D1112_RS09430 (D1112_09510)	2017036..2017401	-	366	WP_052290829.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1112_RS09435 (D1112_09515)	2017464..2017751	-	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D1112_RS09440 (D1112_09520)	2017957..2019672	+	1716	WP_012263406.1	proline--tRNA ligase	-
D1112_RS09445 (D1112_09525)	2019738..2019926	-	189	WP_005602684.1	hypothetical protein	-
D1112_RS09450 (D1112_09530)	2019929..2020510	-	582	WP_005606496.1	sigma-70 family RNA polymerase sigma factor	-
D1112_RS09455 (D1112_09535)	2020599..2021555	+	957	WP_012263408.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
D1112_RS09460 (D1112_09540)	2021555..2022148	+	594	WP_005599453.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13961.01 Da
• Isoelectric Point: 7.7998

>T110380 WP_052290829.1 NZ_CP031854:c2017401-2017036 [Actinobacillus pleuropneumoniae]
IDTNVRQWLETIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQAKSFSINNEIALLASEYHIPNK
MDLNDAYIAATAKYHNLVLVTRNLKDFNCCDIRLFNPFEPN

Nucleotide

• Length: 366 bp

>T110380 NZ_CP031854:c2017401-2017036 [Actinobacillus pleuropneumoniae]
ATAGATACCAATGTACGACAATGGTTAGAAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAAT
AAAAACGGGGATTTTACTTAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTAT
TAAGCGTTTATCAAGCTAAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAA
ATGGATTTAAACGATGCTTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTT
CAACTGTTGCGATATACGTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT110380 WP_005599447.1 NZ_CP031854:c2017751-2017464 [Actinobacillus pleuropneumoniae]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT110380 NZ_CP031854:c2017751-2017464 [Actinobacillus pleuropneumoniae]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5