Detailed information of TA system TA110378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1716903..1717634
• Replicon: chromosome
• Accession: NZ_CP031854
• Organism: Actinobacillus pleuropneumoniae strain ORG1224

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: B0BRD2
• Locus tag: D1112_RS07855
• Protein ID: WP_005605461.1
• Coordinates: 1716903..1717370 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: D1112_RS07860
• Protein ID: WP_005619269.1
• Coordinates: 1717374..1717634 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1112_RS07830 (D1112_07885)	1712258..1712629	-	372	WP_005598830.1	CrcB family protein	-
D1112_RS07835 (D1112_07890)	1712632..1713696	-	1065	WP_011848610.1	MFS transporter	-
D1112_RS07840 (D1112_07895)	1713791..1714900	+	1110	WP_237601053.1	anhydro-N-acetylmuramic acid kinase	-
D1112_RS07845 (D1112_07900)	1714954..1715868	+	915	WP_012263273.1	N-acetylmuramic acid 6-phosphate etherase	-
D1112_RS07850 (D1112_07905)	1715938..1716819	-	882	WP_012263274.1	50S ribosomal protein L11 methyltransferase	-
D1112_RS07855 (D1112_07910)	1716903..1717370	-	468	WP_005605461.1	GNAT family N-acetyltransferase	Toxin
D1112_RS07860 (D1112_07915)	1717374..1717634	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
D1112_RS07865 (D1112_07920)	1717755..1719215	-	1461	WP_012263275.1	metalloprotease TldD	-
D1112_RS07870 (D1112_07925)	1719345..1720604	+	1260	WP_005618010.1	tRNA lysidine(34) synthetase TilS	-
D1112_RS07875 (D1112_07930)	1720631..1720921	-	291	WP_005602215.1	DUF5389 family protein	-
D1112_RS07880 (D1112_07935)	1720923..1721816	-	894	WP_005608892.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (40-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17276.25 Da
• Isoelectric Point: 9.5125

>T110378 WP_005605461.1 NZ_CP031854:c1717370-1716903 [Actinobacillus pleuropneumoniae]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVRDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGVKALLVHALNQQAKQFYLKYGFSCSPIDEMVLMLKL

Nucleotide

• Length: 468 bp

>T110378 NZ_CP031854:c1717370-1716903 [Actinobacillus pleuropneumoniae]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGCGTGATGAGAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGCCATCAGTTTGTTGCAGGCGCATTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGTTCGGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTAGCGAATCAAATCGGGGTAAAAGCATTGCTTGTTCATGCGTTAAATCAGCAAGCAAAAC
AATTTTATTTAAAGTACGGTTTTTCATGTTCACCCATTGATGAGATGGTTTTAATGTTAAAACTTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT110378 WP_005619269.1 NZ_CP031854:c1717634-1717374 [Actinobacillus pleuropneumoniae]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT110378 NZ_CP031854:c1717634-1717374 [Actinobacillus pleuropneumoniae]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGCATTTTAGGTAA
AACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACCGTTTTCCAATTAA
ATGAGCAGGCTTTCGAGCAATTTAACCGATTGCTAGAACAGCCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Toxin

• AlphaFold DB: B0BRD2

Antitoxin

• AlphaFold DB: A3N2I7