Detailed information of TA system TA110377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 1515002..1515647
• Replicon: chromosome
• Accession: NZ_CP031854
• Organism: Actinobacillus pleuropneumoniae strain ORG1224

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: B0BQU7
• Locus tag: D1112_RS06865
• Protein ID: WP_005619674.1
• Coordinates: 1515276..1515647 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: D1112_RS06860
• Protein ID: WP_005598477.1
• Coordinates: 1515002..1515295 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1112_RS06830 (D1112_06885)	1510334..1511017	-	684	WP_237586507.1	arginine ABC transporter permease ArtM	-
D1112_RS06835 (D1112_06890)	1511017..1511688	-	672	WP_005612835.1	arginine ABC transporter permease ArtQ	-
D1112_RS06840 (D1112_06895)	1511694..1512413	-	720	WP_011848574.1	transporter substrate-binding domain-containing protein	-
D1112_RS06845 (D1112_06900)	1512433..1513167	-	735	WP_005598473.1	arginine ABC transporter ATP-binding protein ArtP	-
D1112_RS06850 (D1112_06905)	1513392..1513889	-	498	WP_005601953.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
D1112_RS06855 (D1112_06910)	1513962..1514924	+	963	WP_012263194.1	calcium/sodium antiporter	-
D1112_RS06860 (D1112_06915)	1515002..1515295	-	294	WP_005598477.1	helix-turn-helix domain-containing protein	Antitoxin
D1112_RS06865 (D1112_06920)	1515276..1515647	-	372	WP_005619674.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D1112_RS06870 (D1112_06925)	1515841..1517592	+	1752	WP_043948002.1	protein-disulfide reductase DsbD	-
D1112_RS06875 (D1112_06930)	1517650..1518219	+	570	WP_005605204.1	elongation factor P hydroxylase	-
D1112_RS06880 (D1112_06935)	1518263..1519117	-	855	WP_005598486.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (32-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-85)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 15066.57 Da
• Isoelectric Point: 10.4054

>T110377 WP_005619674.1 NZ_CP031854:c1515647-1515276 [Actinobacillus pleuropneumoniae]
MYEIVFYRDKRGREPVKEFLLRLLKERQEGSRERLHKISHHLSILHLHGTRAGENYIKHLEDRIWQLRPVGDCLLFASII
RGKFVLLHYFAKQNYRIPKREIERAKVRLADLQERIKDEPYWF

Nucleotide

• Length: 372 bp

>T110377 NZ_CP031854:c1515647-1515276 [Actinobacillus pleuropneumoniae]
ATGTATGAAATAGTCTTTTATCGAGATAAGCGGGGGAGAGAACCCGTAAAAGAATTTCTGTTAAGGCTACTAAAAGAACG
GCAGGAAGGCAGTCGAGAACGATTACACAAAATCAGCCATCATTTGAGTATTTTACATTTGCACGGTACTCGAGCCGGTG
AGAATTATATTAAGCATTTGGAAGATCGTATTTGGCAGTTACGTCCGGTGGGCGATTGCCTGTTATTTGCAAGTATTATT
CGGGGAAAATTTGTGTTGTTGCATTATTTTGCCAAACAGAATTATCGAATTCCGAAACGAGAAATAGAACGTGCAAAAGT
TCGGTTGGCGGATTTACAGGAGAGAATAAAAGATGAGCCCTATTGGTTCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10935.81 Da
• Isoelectric Point: 10.3120

>AT110377 WP_005598477.1 NZ_CP031854:c1515295-1515002 [Actinobacillus pleuropneumoniae]
MSPIGSSWTSFEQEIFDESEIAETQFRIRLILEIIETRQQLGISQRKLEKLSGVKQSMIARVEKGSVNPSLATVLKLLVP
LGKTLQIVPLDKNKTVR

Nucleotide

• Length: 294 bp

>AT110377 NZ_CP031854:c1515295-1515002 [Actinobacillus pleuropneumoniae]
ATGAGCCCTATTGGTTCTAGTTGGACATCGTTTGAACAAGAAATTTTTGACGAGTCGGAAATAGCGGAAACTCAGTTTCG
CATACGACTTATTTTAGAAATCATTGAAACCCGTCAGCAGCTCGGTATTTCTCAACGAAAATTGGAGAAATTAAGCGGTG
TAAAACAATCTATGATTGCTCGGGTAGAAAAAGGCTCGGTTAATCCGTCGTTGGCGACGGTATTAAAGTTGCTTGTGCCG
TTGGGGAAAACTTTACAAATCGTACCGTTAGATAAAAACAAAACGGTACGTTAA

Structures

Toxin

• AlphaFold DB: A3N206