Detailed information of TA system TA110376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 1420836..1421344
• Replicon: chromosome
• Accession: NZ_CP031854
• Organism: Actinobacillus pleuropneumoniae strain ORG1224

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B0BQK9
• Locus tag: D1112_RS06380
• Protein ID: WP_005605064.1
• Coordinates: 1421084..1421344 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B0BQK8
• Locus tag: D1112_RS06375
• Protein ID: WP_005598318.1
• Coordinates: 1420836..1421087 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1112_RS06335 (D1112_06390)	1416371..1416919	-	549	WP_012263163.1	type IV pilus biogenesis/stability protein PilW	-
D1112_RS06340 (D1112_06395)	1416973..1418154	-	1182	WP_005608495.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
D1112_RS06365 (D1112_06420)	1419059..1420498	+	1440	WP_005605060.1	glutamate--tRNA ligase	-
D1112_RS06375 (D1112_06430)	1420836..1421087	+	252	WP_005598318.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
D1112_RS06380 (D1112_06435)	1421084..1421344	+	261	WP_005605064.1	Txe/YoeB family addiction module toxin	Toxin
D1112_RS06385 (D1112_06440)	1421502..1421669	-	168	WP_009875287.1	Trm112 family protein	-
D1112_RS06390 (D1112_06445)	1421671..1422651	-	981	WP_005608502.1	tetraacyldisaccharide 4'-kinase	-
D1112_RS06395 (D1112_06450)	1422745..1424004	-	1260	WP_005598324.1	ATP-dependent protease ATP-binding subunit ClpX	-
D1112_RS06400 (D1112_06455)	1424004..1424594	-	591	WP_005598326.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
D1112_RS06405 (D1112_06460)	1424718..1425110	+	393	WP_005618838.1	SufE family protein	-
D1112_RS06420 (D1112_06475)	1425469..1426242	-	774	WP_012263166.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10423.99 Da
• Isoelectric Point: 8.0109

>T110376 WP_005605064.1 NZ_CP031854:1421084-1421344 [Actinobacillus pleuropneumoniae]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKLEPLKFNLSGFWSRRINEEHRLIYSVEDEAILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T110376 NZ_CP031854:1421084-1421344 [Actinobacillus pleuropneumoniae]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9362.66 Da
• Isoelectric Point: 4.5659

>AT110376 WP_005598318.1 NZ_CP031854:1420836-1421087 [Actinobacillus pleuropneumoniae]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYCSLEETAYLMRSPTNAKRLLDSVAELRQGQGIERE
LIE

Nucleotide

• Length: 252 bp

>AT110376 NZ_CP031854:1420836-1421087 [Actinobacillus pleuropneumoniae]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACGATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TGATGCGTTCACCCACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Toxin

• AlphaFold DB: B0BQK9

Antitoxin

• AlphaFold DB: B0BQK8