Detailed information of TA system TA110375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 725346..725996
• Replicon: chromosome
• Accession: NZ_CP031854
• Organism: Actinobacillus pleuropneumoniae strain ORG1224

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B0BNU3
• Locus tag: D1112_RS03240
• Protein ID: WP_005614926.1
• Coordinates: 725346..725735 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BNU4
• Locus tag: D1112_RS03245
• Protein ID: WP_005604117.1
• Coordinates: 725736..725996 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1112_RS03230 (D1112_03240)	720586..723861	-	3276	WP_012262906.1	helicase-related protein	-
D1112_RS03235 (D1112_03245)	723987..725210	-	1224	WP_012262907.1	ATP-binding protein	-
D1112_RS03240 (D1112_03250)	725346..725735	-	390	WP_005614926.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1112_RS03245 (D1112_03255)	725736..725996	-	261	WP_005604117.1	hypothetical protein	Antitoxin
D1112_RS03250 (D1112_03260)	726342..727178	+	837	WP_012262908.1	DUF6216 family protein	-
D1112_RS03255 (D1112_03265)	727210..729231	-	2022	WP_012262909.1	excinuclease ABC subunit UvrB	-
D1112_RS03260 (D1112_03275)	729408..730220	+	813	WP_005607533.1	iron uptake system protein EfeO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14837.17 Da
• Isoelectric Point: 5.1295

>T110375 WP_005614926.1 NZ_CP031854:c725735-725346 [Actinobacillus pleuropneumoniae]
MYMLDTNTVSYFFRQDPTVVKKLQQLNPELICISSVTAAELFYGVKKRNNQKLTAFLNTFLSAITVMDWDYQVAEVYGQL
RAEMEREGKIMGVQDQMIGAHALATECVLVSSDKAFQFIPNLVLENWWK

Nucleotide

• Length: 390 bp

>T110375 NZ_CP031854:c725735-725346 [Actinobacillus pleuropneumoniae]
ATGTATATGCTAGATACTAATACGGTGAGTTATTTCTTTAGGCAAGATCCTACTGTGGTTAAGAAACTACAACAACTTAA
TCCGGAATTGATTTGTATTTCATCGGTCACGGCTGCAGAGTTGTTTTATGGCGTAAAAAAACGTAATAATCAGAAACTTA
CTGCATTTTTAAATACTTTTTTATCTGCGATTACGGTGATGGATTGGGATTATCAAGTGGCGGAAGTTTATGGTCAGCTG
CGAGCAGAGATGGAACGAGAAGGCAAAATCATGGGCGTTCAAGATCAAATGATTGGAGCTCATGCCCTAGCAACAGAATG
TGTTCTTGTTTCCAGCGATAAAGCCTTTCAATTTATCCCTAATCTCGTATTAGAAAATTGGTGGAAGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10289.63 Da
• Isoelectric Point: 5.1867

>AT110375 WP_005604117.1 NZ_CP031854:c725996-725736 [Actinobacillus pleuropneumoniae]
MEKVARLFRNGRNQAVRLPVEFEFNAKQVYVFKEANGDITLSTRSRNQQNWQKVFELLPMLECDEQFLSEQERKQEMATR
DPFEGF

Nucleotide

• Length: 261 bp

>AT110375 NZ_CP031854:c725996-725736 [Actinobacillus pleuropneumoniae]
ATGGAAAAGGTCGCACGTTTATTTAGAAACGGACGTAATCAAGCCGTACGATTACCGGTTGAATTTGAGTTTAATGCGAA
GCAAGTTTATGTGTTTAAAGAAGCAAACGGCGATATTACGCTTTCGACACGTTCTCGCAATCAGCAAAATTGGCAGAAAG
TCTTTGAGTTATTACCAATGCTTGAATGTGATGAACAGTTTTTATCGGAACAAGAACGCAAGCAAGAAATGGCAACTCGA
GATCCGTTCGAGGGCTTTTAA

Structures

Toxin

• AlphaFold DB: A3N029

Antitoxin

• AlphaFold DB: A3N030