Detailed information of TA system TA110367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 4385..5028
• Replicon: plasmid pKP121-2
• Accession: NZ_CP031851
• Organism: Klebsiella pneumoniae strain 121

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: D1Y71_RS27325
• Protein ID: WP_048335684.1
• Coordinates: 4612..5028 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: D1Y71_RS27320
• Protein ID: WP_001261276.1
• Coordinates: 4385..4615 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1Y71_RS27285	1..1011	+	1011	WP_136398500.1	RepB family plasmid replication initiator protein	-
D1Y71_RS27295	1716..2456	-	741	WP_136398501.1	tyrosine-type recombinase/integrase	-
D1Y71_RS27305	3105..3371	-	267	WP_024144810.1	type II toxin-antitoxin system ParD family antitoxin	-
D1Y71_RS27310	3451..3813	-	363	WP_115658248.1	hypothetical protein	-
D1Y71_RS27320	4385..4615	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D1Y71_RS27325	4612..5028	+	417	WP_048335684.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1Y71_RS27335	6216..6911	+	696	WP_048335678.1	membrane protein	-
D1Y71_RS27340	6930..8222	+	1293	WP_114507397.1	histidine-type phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..134208	134208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15079.57 Da
• Isoelectric Point: 7.8644

>T110367 WP_048335684.1 NZ_CP031851:4612-5028 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLTLEDWVI

Nucleotide

• Length: 417 bp

>T110367 NZ_CP031851:4612-5028 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCGTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCCGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT110367 WP_001261276.1 NZ_CP031851:4385-4615 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT110367 NZ_CP031851:4385-4615 [Klebsiella pneumoniae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023