Detailed information of TA system TA110348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 199618..200357
• Replicon: chromosome
• Accession: NZ_CP031849
• Organism: Klebsiella pneumoniae strain 121

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: D1Y71_RS01005
• Protein ID: WP_021312536.1
• Coordinates: 199872..200357 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: D1Y71_RS01000
• Protein ID: WP_003026799.1
• Coordinates: 199618..199884 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1Y71_RS00985	195122..197191	+	2070	WP_002922127.1	glycine--tRNA ligase subunit beta	-
D1Y71_RS00990	197487..198856	+	1370	WP_087661561.1	IS3 family transposase	-
D1Y71_RS00995	199057..199485	+	429	WP_004901287.1	GFA family protein	-
D1Y71_RS01000	199618..199884	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
D1Y71_RS01005	199872..200357	+	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
D1Y71_RS01010	200701..200853	+	153	WP_002922102.1	type I toxin-antitoxin system toxin HokA	-
D1Y71_RS01015	201155..202774	+	1620	WP_136398022.1	ATP-binding cassette domain-containing protein	-
D1Y71_RS01020	202873..203085	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
D1Y71_RS01025	203337..203627	-	291	WP_002921931.1	HTH-type transcriptional regulator	-
D1Y71_RS01030	203873..205228	-	1356	WP_032412370.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	198131..198856	725

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T110348 WP_021312536.1 NZ_CP031849:199872-200357 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T110348 NZ_CP031849:199872-200357 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT110348 WP_003026799.1 NZ_CP031849:199618-199884 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT110348 NZ_CP031849:199618-199884 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66