Detailed information of TA system TA110328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3199645..3200666
• Replicon: chromosome
• Accession: NZ_CP031846
• Organism: Proteus mirabilis strain XH983

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: D1J70_RS15170
• Protein ID: WP_004249488.1
• Coordinates: 3200097..3200666 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: D1J70_RS15165
• Protein ID: WP_004245528.1
• Coordinates: 3199645..3200097 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1J70_RS15145 (D1J70_15200)	3195431..3195961	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
D1J70_RS15150 (D1J70_15205)	3196278..3197243	+	966	WP_049195511.1	thiamine ABC transporter substrate binding subunit	-
D1J70_RS15155 (D1J70_15210)	3197243..3198823	+	1581	WP_004245526.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
D1J70_RS15160 (D1J70_15215)	3198816..3199517	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
D1J70_RS15165 (D1J70_15220)	3199645..3200097	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
D1J70_RS15170 (D1J70_15225)	3200097..3200666	+	570	WP_004249488.1	PIN domain-containing protein	Toxin
D1J70_RS15175 (D1J70_15230)	3200782..3203136	+	2355	WP_017628571.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21600.10 Da
• Isoelectric Point: 7.3470

>T110328 WP_004249488.1 NZ_CP031846:3200097-3200666 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKNPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T110328 NZ_CP031846:3200097-3200666 [Proteus mirabilis]
ATGACAAACTATACTGTTTTGCTTGATGCTTGTGTCATGTATCCAGCCCCTCTTAGAAGTTATTTAATGTATCTTGCTAA
TACTGGTTTATTTAGAGCACGTTGGAGTAATCAGATCCATGATGAATGGATCACTAATTTATTAGTCAACAATCCAACAT
TAACAGCTGAGCGACTAATACGAACCAGAGATTTAATGAATGCACATGTTCCTGATTGTTTAGTAGAAGGCTACGAACCG
CTTATTTGTGGTTTAAAGCTACCTGATCCTAATGATCGTCATGTGCTAGCAGCAGCAATAAAAGGGCAGGCTCAAGCTAT
AATTACATTTAACTTAAAAGATTTTCCGCAAAAATACCTTAATTCTTTTGATATTTCAGCTATCCATCCTGATGAATTTT
TGAGTGACATGTTTGAATTAAATCCTAGTAGCTGTATTAAAGCGGCGCAACAACATAGACAAAGCCTAAAGAATCCACCA
ATGACCGTAGAGACCTTTTTAGGCTGTTTACAAAAGCAGAAATTACCCCAGTTTATTTCCCATTTACGGGCATTCCAGAT
ATTATTATAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT110328 WP_004245528.1 NZ_CP031846:3199645-3200097 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT110328 NZ_CP031846:3199645-3200097 [Proteus mirabilis]
ATGAATACGATAAGTGAGAGACTTAGTGCTGAAGATATTGCATTAGCAAAATTAAGTAGCCAAGAGCTATCCGCTGTTAT
GGATATTAATGGCAAAACGCAGAAGCTATCCATTATAGATAAGACGGGTAATAATCATATTGTACAAATACCAAATAGTG
CATTACGCCTAATGATTGAAATTCTTACTCAACTAGGGCAAGGGAATACTGTTAGTATCACACCAATCCATGCTGAGCTA
ACGACACAGGAAGCGGCTGATATACTCAATATGTCCCGTCCTACATTTGTAAAATTATTAGATACACGAGCGATACCCTA
CTCTAGAAAAGGAAATCGTAGGAAAGTTGCTTATGCTGATATTGTTGCTTATAAAAATGAATTGGAGCAAAAACGACTTG
ATGCCCTTGCTGAATTATCGCAATTAGATCAAGAAATGGATATGGGGTATTAA

Structures

Antitoxin

• AlphaFold DB: B4F2H3