Detailed information of TA system TA110327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1753830..1754629
• Replicon: chromosome
• Accession: NZ_CP031846
• Organism: Proteus mirabilis strain XH983

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A8E3RD10
• Locus tag: D1J70_RS08440
• Protein ID: WP_004247966.1
• Coordinates: 1754105..1754629 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: B4EVS2
• Locus tag: D1J70_RS08435
• Protein ID: WP_004247964.1
• Coordinates: 1753830..1754108 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1J70_RS08410 (D1J70_08420)	1749180..1750262	+	1083	WP_004242680.1	peptide chain release factor 1	-
D1J70_RS08415 (D1J70_08425)	1750262..1751110	+	849	WP_004247962.1	peptide chain release factor N(5)-glutamine methyltransferase	-
D1J70_RS08420 (D1J70_08430)	1751088..1751903	+	816	WP_004242688.1	invasion regulator SirB1	-
D1J70_RS08425 (D1J70_08435)	1751961..1752815	+	855	WP_004242689.1	3-deoxy-8-phosphooctulonate synthase	-
D1J70_RS08430 (D1J70_08440)	1752823..1753752	+	930	WP_017628160.1	TIGR01212 family radical SAM protein	-
D1J70_RS08435 (D1J70_08445)	1753830..1754108	+	279	WP_004247964.1	DUF1778 domain-containing protein	Antitoxin
D1J70_RS08440 (D1J70_08450)	1754105..1754629	+	525	WP_004247966.1	hypothetical protein	Toxin
D1J70_RS08445 (D1J70_08455)	1754710..1756410	-	1701	WP_017628159.1	C4-dicarboxylic acid transporter DauA	-
D1J70_RS08455 (D1J70_08465)	1757374..1758315	+	942	WP_004242695.1	glyoxylate/hydroxypyruvate reductase GhrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (5-87)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19192.12 Da
• Isoelectric Point: 9.3561

>T110327 WP_004247966.1 NZ_CP031846:1754105-1754629 [Proteus mirabilis]
MTIPNWHEEAISKKHNRNGFNCGDAVLNQFLYRHARQNHENGSAKTYLAINNSNNIILGYYSLCPASIEFERTPELIKHG
LARHDIPVFKLARLATDLSVQGKGLGGQLLLAAGRRCLSVASELGGVALLIDAKNERVANWYISYGAIPLLDTPLSLLIS
FKTIYTALSMANKI

Nucleotide

• Length: 525 bp

>T110327 NZ_CP031846:1754105-1754629 [Proteus mirabilis]
ATGACAATACCAAATTGGCACGAAGAAGCGATCAGTAAAAAACATAATAGAAATGGATTTAATTGTGGCGATGCAGTACT
AAACCAATTTCTGTATCGCCATGCAAGACAAAATCATGAGAATGGTAGTGCGAAAACATATTTAGCTATCAATAATAGTA
ATAATATTATATTAGGTTATTACAGCTTATGTCCTGCTTCTATTGAGTTCGAAAGAACGCCCGAATTGATCAAACATGGC
TTAGCGCGTCATGATATCCCTGTTTTTAAACTTGCACGACTAGCTACGGATCTATCCGTTCAAGGAAAAGGTTTGGGTGG
GCAATTATTGCTTGCAGCAGGAAGGCGTTGTCTTTCCGTTGCTAGTGAATTGGGAGGCGTTGCTTTACTTATAGATGCTA
AAAATGAACGAGTCGCTAATTGGTATATTAGCTACGGGGCAATACCATTATTAGATACCCCTCTTTCTTTATTAATTTCC
TTTAAAACCATTTATACTGCACTTTCAATGGCAAATAAAATATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10252.82 Da
• Isoelectric Point: 5.1369

>AT110327 WP_004247964.1 NZ_CP031846:1753830-1754108 [Proteus mirabilis]
MAQALVENSERVSIRITTEDKSLLVRAASLENTNLTEFVLRNIVPVARQVIENNERLKLSEKDTLRVMELLDNPPKPNAK
LLAAAFALPDNA

Nucleotide

• Length: 279 bp

>AT110327 NZ_CP031846:1753830-1754108 [Proteus mirabilis]
ATGGCTCAAGCACTAGTAGAAAACAGTGAACGAGTGTCAATTCGTATCACTACAGAAGATAAATCACTGCTGGTTCGTGC
TGCTAGTTTGGAAAATACAAACTTAACAGAGTTTGTACTTCGTAATATTGTACCTGTAGCTCGGCAAGTTATTGAAAATA
ATGAGCGTTTAAAATTATCAGAAAAAGACACATTACGTGTTATGGAACTTTTAGATAATCCACCAAAGCCTAATGCTAAA
CTACTTGCAGCGGCTTTTGCTCTACCGGACAATGCATGA

Structures

Antitoxin

• AlphaFold DB: B4EVS2