Detailed information of TA system TA110326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1478319..1478964
• Replicon: chromosome
• Accession: NZ_CP031846
• Organism: Proteus mirabilis strain XH983

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B4ET86
• Locus tag: D1J70_RS06965
• Protein ID: WP_004247035.1
• Coordinates: 1478779..1478964 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B4ET85
• Locus tag: D1J70_RS06960
• Protein ID: WP_004247034.1
• Coordinates: 1478319..1478732 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1J70_RS06930	1473872..1474030	-	159	WP_004247025.1	hypothetical protein	-
D1J70_RS06935	1474482..1474658	+	177	WP_004247793.1	hypothetical protein	-
D1J70_RS06940 (D1J70_06940)	1474829..1475575	-	747	Protein_1332	methyltransferase domain-containing protein	-
D1J70_RS06945 (D1J70_06950)	1476425..1477003	+	579	WP_017628400.1	alpha/beta hydrolase	-
D1J70_RS06950 (D1J70_06955)	1477116..1477358	-	243	WP_230458803.1	hypothetical protein	-
D1J70_RS06955 (D1J70_06965)	1477723..1478223	-	501	WP_004247032.1	response regulator	-
D1J70_RS06960 (D1J70_06970)	1478319..1478732	-	414	WP_004247034.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D1J70_RS06965 (D1J70_06975)	1478779..1478964	-	186	WP_004247035.1	type II toxin-antitoxin system HicA family toxin	Toxin
D1J70_RS06970	1479040..1479537	+	498	WP_223852280.1	DUF1073 domain-containing protein	-
D1J70_RS06975	1479480..1479848	+	369	WP_223293455.1	DUF1073 domain-containing protein	-
D1J70_RS06980 (D1J70_06985)	1479854..1480075	+	222	Protein_1340	hypothetical protein	-
D1J70_RS06985 (D1J70_06990)	1480404..1480955	-	552	WP_017628396.1	PH domain-containing protein	-
D1J70_RS06990 (D1J70_06995)	1481019..1481333	-	315	WP_017628395.1	hypothetical protein	-
D1J70_RS07000 (D1J70_07005)	1481740..1483473	-	1734	WP_017628394.1	LPS biosynthesis-modulating metalloenzyme YejM	-
D1J70_RS07005 (D1J70_07010)	1483497..1483724	-	228	WP_004247042.1	YejL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1475577..1490946	15369

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 7049.30 Da
• Isoelectric Point: 10.9659

>T110326 WP_004247035.1 NZ_CP031846:c1478964-1478779 [Proteus mirabilis]
MKSSELIKLLEKNGWILDRIKGSHHQFTHPDFSFVVTVPHPRKDLKKGTLNQIIKSAKLKN

Nucleotide

• Length: 186 bp

>T110326 NZ_CP031846:c1478964-1478779 [Proteus mirabilis]
TTGAAAAGTTCGGAACTGATTAAGTTGTTAGAAAAAAACGGATGGATACTTGATAGAATAAAAGGAAGTCATCATCAATT
TACTCACCCTGATTTTTCTTTTGTTGTAACAGTACCGCATCCAAGAAAAGATTTAAAAAAAGGAACATTAAATCAAATTA
TTAAAAGTGCCAAACTGAAAAATTAA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15251.98 Da
• Isoelectric Point: 4.3830

>AT110326 WP_004247034.1 NZ_CP031846:c1478732-1478319 [Proteus mirabilis]
MLYPAFIEIDEDGSASGWFPDIDGCIFAGDSIEEAHADARSAIDAHFELLSEKGLDIPSPKSQQEHLINSSGDYNNGIWL
LVDVDMDKYDGRAERINITLPHRLLSRIDTIVKTNPEYGSRSGFIATATRKELQKNI

Nucleotide

• Length: 414 bp

>AT110326 NZ_CP031846:c1478732-1478319 [Proteus mirabilis]
ATGTTATATCCAGCCTTTATTGAAATTGATGAAGATGGTAGCGCAAGTGGCTGGTTCCCTGATATTGATGGTTGCATCTT
TGCGGGGGACAGTATTGAAGAAGCTCACGCAGATGCGAGGAGCGCTATTGATGCCCATTTTGAATTGTTAAGCGAGAAGG
GTTTAGATATCCCCTCTCCAAAATCTCAACAAGAGCATTTAATTAATTCTAGTGGTGATTACAATAATGGAATATGGCTT
TTAGTTGATGTTGATATGGATAAATACGACGGACGAGCAGAGCGTATTAATATCACATTGCCACATCGATTATTGAGTCG
CATCGATACTATCGTAAAAACAAATCCTGAATATGGGAGTCGTAGCGGATTTATTGCCACAGCAACCCGTAAAGAACTCC
AAAAGAATATTTAA

Structures

Toxin

• AlphaFold DB: B4ET86

Antitoxin

• AlphaFold DB: B4ET85