Detailed information of TA system TA110306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 717959..718596
• Replicon: chromosome
• Accession: NZ_CP031841
• Organism: Breoghania sp. L-A4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: D1F64_RS03460
• Protein ID: WP_117411282.1
• Coordinates: 718342..718596 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: D1F64_RS03455
• Protein ID: WP_117411281.1
• Coordinates: 717959..718330 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1F64_RS03425	713506..713769	+	264	WP_162901261.1	phage tail assembly protein	-
D1F64_RS23035	713941..714099	+	159	WP_162901262.1	hypothetical protein	-
D1F64_RS03430	714251..716068	+	1818	WP_117411276.1	phage tail tape measure protein	-
D1F64_RS03435	716068..716268	+	201	WP_117411277.1	hypothetical protein	-
D1F64_RS03440	716258..716722	+	465	WP_117411278.1	phage tail protein	-
D1F64_RS03445	716739..716951	+	213	WP_117411279.1	tail protein X	-
D1F64_RS03450	716955..717950	+	996	WP_117411280.1	late control protein D	-
D1F64_RS03455	717959..718330	-	372	WP_117411281.1	helix-turn-helix domain-containing protein	Antitoxin
D1F64_RS03460	718342..718596	-	255	WP_117411282.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D1F64_RS03465	718748..719530	+	783	WP_117411283.1	peptidoglycan-binding domain-containing protein	-
D1F64_RS03470	719574..720056	+	483	WP_117411284.1	hypothetical protein	-
D1F64_RS03475	720222..720476	+	255	WP_117411285.1	hypothetical protein	-
D1F64_RS03480	720469..720795	+	327	WP_117411286.1	hypothetical protein	-
D1F64_RS03485	721077..721907	+	831	WP_117411287.1	DNA adenine methylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	660290..729614	69324

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9225.71 Da
• Isoelectric Point: 11.2577

>T110306 WP_117411282.1 NZ_CP031841:c718596-718342 [Breoghania sp. L-A4]
MKQIAYSKGATRTLSRMPANVARNIRSKIEQYASTPESLAGNVKTLKGMDGIFRLRVGDWRVIFSEAGDVIAIIRIAPRG
GAYN

Nucleotide

• Length: 255 bp

>T110306 NZ_CP031841:c718596-718342 [Breoghania sp. L-A4]
ATGAAACAGATCGCCTACAGCAAGGGCGCGACCAGGACCTTGAGCCGGATGCCGGCCAACGTCGCGCGCAATATCCGCTC
CAAGATCGAGCAATACGCGAGCACTCCTGAAAGCCTTGCCGGCAACGTGAAGACGCTCAAGGGCATGGACGGCATCTTTC
GCCTGCGCGTCGGCGACTGGCGGGTGATCTTCTCAGAGGCCGGCGACGTCATCGCCATCATCAGGATCGCGCCGCGCGGC
GGCGCCTACAACTAG

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13396.11 Da
• Isoelectric Point: 4.2779

>AT110306 WP_117411281.1 NZ_CP031841:c718330-717959 [Breoghania sp. L-A4]
MSEQIITTPAGERLVVLPEDEYLAMREALDDRADIEAVRAFEHKLAAGEEELIPAEFVNRILDGENKVRVWRDYRGLTAR
DLAAAAGLSAAYVSEIETNKKDGSVAALKAIAAALRADLDDIA

Nucleotide

• Length: 372 bp

>AT110306 NZ_CP031841:c718330-717959 [Breoghania sp. L-A4]
ATGTCCGAACAGATCATCACCACTCCCGCGGGCGAGCGCCTCGTCGTGCTGCCCGAGGACGAATATCTCGCCATGCGCGA
AGCGCTGGACGACCGCGCGGATATCGAGGCCGTGCGCGCGTTTGAGCACAAGCTCGCCGCTGGCGAGGAGGAGCTTATCC
CGGCCGAATTCGTCAACCGCATCCTCGACGGCGAGAACAAGGTGCGCGTCTGGCGCGATTATCGCGGGCTTACTGCCCGT
GACCTCGCTGCCGCCGCCGGCCTGAGTGCTGCCTATGTCTCCGAGATCGAGACGAACAAGAAGGACGGGAGCGTCGCCGC
GCTGAAGGCGATCGCCGCAGCGCTACGCGCCGACCTCGACGACATCGCCTGA