Detailed information of TA system TA110281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 722876..723405
• Replicon: chromosome
• Accession: NZ_CP031838
• Organism: Staphylococcus aureus strain QD-CD9

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: D1G35_RS03780
• Protein ID: WP_000621175.1
• Coordinates: 723043..723405 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: D1G35_RS03775
• Protein ID: WP_000948331.1
• Coordinates: 722876..723046 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1G35_RS03745	717912..718472	+	561	WP_001092410.1	K(+)-transporting ATPase subunit C	-
D1G35_RS03750	718681..719160	+	480	WP_031590204.1	hypothetical protein	-
D1G35_RS03755	719153..720736	+	1584	WP_001294626.1	PH domain-containing protein	-
D1G35_RS03760	720723..721214	+	492	WP_072353916.1	PH domain-containing protein	-
D1G35_RS03765	721218..721577	+	360	WP_000581197.1	holo-ACP synthase	-
D1G35_RS03770	721643..722791	+	1149	WP_001281154.1	alanine racemase	-
D1G35_RS03775	722876..723046	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
D1G35_RS03780	723043..723405	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D1G35_RS03790	723755..724756	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
D1G35_RS03795	724875..725201	+	327	WP_001052491.1	anti-sigma factor antagonist	-
D1G35_RS03800	725203..725682	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
D1G35_RS03805	725657..726427	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T110281 WP_000621175.1 NZ_CP031838:723043-723405 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T110281 NZ_CP031838:723043-723405 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT110281 WP_000948331.1 NZ_CP031838:722876-723046 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT110281 NZ_CP031838:722876-723046 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1