Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3531705..3532323 Replicon chromosome
Accession NZ_CP031833
Organism Escherichia coli strain 2452

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag D0R13_RS18205 Protein ID WP_001291435.1
Coordinates 3532105..3532323 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag D0R13_RS18200 Protein ID WP_000344800.1
Coordinates 3531705..3532079 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0R13_RS18190 3526794..3527987 + 1194 WP_094319299.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
D0R13_RS18195 3528010..3531159 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
D0R13_RS18200 3531705..3532079 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
D0R13_RS18205 3532105..3532323 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
D0R13_RS18210 3532495..3533046 + 552 WP_000102564.1 maltose O-acetyltransferase -
D0R13_RS18215 3533162..3533632 + 471 WP_000136192.1 YlaC family protein -
D0R13_RS18220 3533796..3535346 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
D0R13_RS18225 3535388..3535741 - 354 WP_000878140.1 DUF1428 domain-containing protein -
D0R13_RS18235 3536120..3536431 + 312 WP_000409911.1 MGMT family protein -
D0R13_RS18240 3536462..3537034 - 573 WP_117355301.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T110265 WP_001291435.1 NZ_CP031833:3532105-3532323 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T110265 NZ_CP031833:3532105-3532323 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT110265 WP_000344800.1 NZ_CP031833:3531705-3532079 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT110265 NZ_CP031833:3531705-3532079 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References