Detailed information of TA system TA110244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 210680..211590
• Replicon: plasmid unnamed1
• Accession: NZ_CP031818
• Organism: Klebsiella pneumoniae strain INF235-sc-2280127

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: D0898_RS26710
• Protein ID: WP_110242314.1
• Coordinates: 210680..211150 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: D0898_RS26715
• Protein ID: WP_004181895.1
• Coordinates: 211147..211590 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0898_RS26695	206897..208429	+	1533	WP_009310015.1	IS3-like element ISKpn38 family transposase	-
D0898_RS26700	208558..209739	+	1182	WP_004883380.1	IS481 family transposase	-
D0898_RS26705	210310..210570	+	261	WP_004026352.1	hypothetical protein	-
D0898_RS26710	210680..211150	-	471	WP_110242314.1	RES family NAD+ phosphorylase	Toxin
D0898_RS26715	211147..211590	-	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
D0898_RS26720	211691..212866	-	1176	Protein_210	translesion error-prone DNA polymerase V subunit UmuC	-
D0898_RS26725	213009..213467	+	459	Protein_211	recombinase family protein	-
D0898_RS26730	213718..213927	+	210	WP_072072310.1	microcin B17	-
D0898_RS26735	213986..214840	+	855	WP_048268726.1	McbB family protein	-
D0898_RS26740	214842..215648	+	807	WP_048268727.1	SagB/ThcOx family dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..430829	430829
	-	inside	IScluster/Tn	-	-	206897..213467	6570

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17529.82 Da
• Isoelectric Point: 4.6155

>T110244 WP_110242314.1 NZ_CP031818:c211150-210680 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRTSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T110244 NZ_CP031818:c211150-210680 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAC
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT110244 WP_004181895.1 NZ_CP031818:c211590-211147 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT110244 NZ_CP031818:c211590-211147 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2J4RLY9