Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 181029..181555 Replicon plasmid unnamed1
Accession NZ_CP031818
Organism Klebsiella pneumoniae strain INF235-sc-2280127

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag D0898_RS26545 Protein ID WP_000323025.1
Coordinates 181029..181316 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag D0898_RS26550 Protein ID WP_000534858.1
Coordinates 181316..181555 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0898_RS26525 177542..177919 + 378 WP_045289277.1 hypothetical protein -
D0898_RS29165 178117..179091 - 975 WP_174232731.1 Hok/Gef family protein -
D0898_RS26535 179698..179856 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
D0898_RS26540 179928..180962 + 1035 Protein_176 IS481 family transposase -
D0898_RS26545 181029..181316 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
D0898_RS26550 181316..181555 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
D0898_RS26555 181952..182968 + 1017 WP_001310555.1 IS5-like element IS5 family transposase -
D0898_RS26560 183345..184082 - 738 WP_004201234.1 hypothetical protein -
D0898_RS26565 184220..184906 - 687 WP_004201232.1 NYN domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..430829 430829
- inside IScluster/Tn - - 179928..182968 3040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T110243 WP_000323025.1 NZ_CP031818:c181316-181029 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T110243 NZ_CP031818:c181316-181029 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT110243 WP_000534858.1 NZ_CP031818:c181555-181316 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT110243 NZ_CP031818:c181555-181316 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References