Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5067865..5068490 Replicon chromosome
Accession NZ_CP031817
Organism Klebsiella pneumoniae strain INF235-sc-2280127

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag D0898_RS25180 Protein ID WP_002882817.1
Coordinates 5067865..5068248 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag D0898_RS25185 Protein ID WP_004150355.1
Coordinates 5068248..5068490 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0898_RS25165 5065231..5066133 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
D0898_RS25170 5066130..5066765 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
D0898_RS25175 5066762..5067691 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
D0898_RS25180 5067865..5068248 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
D0898_RS25185 5068248..5068490 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
D0898_RS25190 5068695..5069612 + 918 WP_004178029.1 alpha/beta hydrolase -
D0898_RS25195 5069626..5070567 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
D0898_RS25200 5070612..5071049 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
D0898_RS25205 5071046..5071906 - 861 WP_002882807.1 virulence factor BrkB family protein -
D0898_RS25210 5071900..5072499 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T110242 WP_002882817.1 NZ_CP031817:c5068248-5067865 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T110242 NZ_CP031817:c5068248-5067865 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT110242 WP_004150355.1 NZ_CP031817:c5068490-5068248 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT110242 NZ_CP031817:c5068490-5068248 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References