Detailed information of TA system TA110221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 20263..21173
• Replicon: plasmid unnamed1
• Accession: NZ_CP031815
• Organism: Klebsiella pneumoniae strain KSB1_7F-sc-2280268

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: D0899_RS26690
• Protein ID: WP_032413375.1
• Coordinates: 20703..21173 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: D0899_RS26685
• Protein ID: WP_032413443.1
• Coordinates: 20263..20706 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0899_RS26650	16097..17095	+	999	WP_032413381.1	N(2)-acetyl-L-2,4-diaminobutanoate deacetylase DoeB	-
D0899_RS26655	17142..18320	+	1179	WP_032413379.1	cystathionine gamma-synthase family protein	-
D0899_RS26660	18457..18804	+	348	WP_032413377.1	hypothetical protein	-
D0899_RS26665	18862..18987	+	126	Protein_19	transposase	-
D0899_RS26670	19121..19345	-	225	Protein_20	transposase	-
D0899_RS26675	19365..19550	+	186	WP_117387360.1	DUF2188 domain-containing protein	-
D0899_RS26680	19651..20163	+	513	Protein_22	DUF4113 domain-containing protein	-
D0899_RS26685	20263..20706	+	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
D0899_RS26690	20703..21173	+	471	WP_032413375.1	RES family NAD+ phosphorylase	Toxin
D0899_RS26695	21283..21543	-	261	WP_017901409.1	hypothetical protein	-
D0899_RS26700	22115..22492	-	378	Protein_26	transposase	-
D0899_RS26705	22626..23594	+	969	WP_162887377.1	IS5 family transposase	-
D0899_RS26710	23829..25562	-	1734	WP_117387351.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..139276	139276
	-	flank	IS/Tn	-	-	22671..23594	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17547.79 Da
• Isoelectric Point: 4.5152

>T110221 WP_032413375.1 NZ_CP031815:20703-21173 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIAFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T110221 NZ_CP031815:20703-21173 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATAGCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT110221 WP_032413443.1 NZ_CP031815:20263-20706 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT110221 NZ_CP031815:20263-20706 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATAAATGAAGGTCTGGACGGGAAGGTGGCGAACCGGATAACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4